10-MDP-钙盐形成对牙本质粘接成绩影响的评价

目的　分析10-MDP-钙盐形成对牙本质粘接成绩的影响。方法　采用酸蚀冲洗粘接模式，根据牙本质表面的处理方式和选择粘接剂的不同将牙齿随机分为以下4组（n=5）进行处理，制作牙本质/树脂粘接试件：①对照组，直接使用全酸蚀粘接剂Single bond 2(SB2)处理后粘接；②10-MDP组，使用SB2处理进行粘接前，牙本质表面以含有磷酸酯单体10-MDP的自配底涂剂预处理；③CHX组，使用SB2处理进行粘接前，先以氯己定（CHX）预处理牙本质表面;④SBU组，使用包含10-MDP的通用型粘接剂Single bond universal(SBU)处理后进行粘接。通过微拉伸测试（μTBS）测试粘接强度，以X射线衍射(XRD)、原位酶谱测试表征自配10-MDP底涂剂和两种牙本质粘接剂处理的牙本质表面，分析10-MDP-钙盐形成对牙本质粘接成绩的影响。结果　微拉伸结果显示，不同处理方式的粘接试件在24 h水储后没有表现出明显的统计学差异(P>0.1)；经过6个月的水储后，与10-MDP组和SBU组相比，对照组的微拉伸强度显著降低(P<0.05)，而CHX组的微拉伸强度没有明显变化(P>0.05)。XRD结果显示,在10-MDP组和SBU组均检测到10-MDP-钙盐形成的特征性峰，表明有10-MDP-钙盐的形成。原位酶谱结果显示，10-MDP组与SBU组之间混合层荧光强度没有明显区别，但均明显高于对照组，CHX组荧光强度低于10-MDP组与SBU组。结论　10-MDP-钙盐的形成能够保护暴露的胶原纤维不接触到MMPs而免于水解，从而增强牙本质/树脂的粘接成绩。

Evaluation of the effect of 10-MDP-calcium salt formation on dentin bonding performance

Objective To analyze the effect of 10-MDP-calcium salt formation on dentin bonding performance.Methods Using the etch-and-rinse technique mode,the teeth were randomly divided into the following 4 groups(n=5)according to the treatment method of the dentin surface and the choice of the adhesive,and the dentin/resin bonding specimens were made:(1)in the control group,the single bond 2(SB2)was directly treated and bonded;(2)in the 10-MDP group,the dentin surface was pretreated with a self-prepared primer containing phosphate monomer 10-MDP before using SB2 treatment for bonding;(3)in the CHX group,the dentin surface was pretreated with chlorhexidine(CHX)before bonding with SB2;(4)in the SBU group,single bond universal(SBU),a MDP-containing universal adhesive,was directly used for bonding.The bonding strength was measured by micro-tensile bond strength(μTBS),and the dentin surface treated with self-prepared 10-MDP primer and two kinds of dentine adhesives was characterized by X-ray diffraction(XRD)and in situ zymography test to analyze the effect of 10-MDP-calcium salt formation on the dentin bonding performance.Results The results of micro-tensile bond strength test showed that there was no significant statistical difference between the adhesive specimens treated with different methods after 24 h water-storage(P>0.1).After 6 months water-storage,compared with the 10-MDP group and the SBU group,the micro-tensile strength test of the control group was significantly reduced(P<0.05),while the micro-tensile strength test of the CHX group did not change significantly(P>0.05).The XRD results showed that the characteristic peaks of 10-MDP-calcium salt formation were detected in both the 10-MDP group and the SBU group,indicating the formation of 10-MDPcalcium salt.The results of in situ zymography showed that there was no significant difference between the 10-MDP group and the SBU group.The fluorescence intensity of the hybrid layers in the control group was significantly higher than that of the 10-MDP group and the SBU group,while the fluorescence intensity of the CHX group was lower than that of the 10-MDP group and the SBU group.Conclusion The formation of 10-MDP-calcium salt can protect the exposed collagen fibers from contacting MMPs and avoid hydrolysis,thereby enhancing the dentin/resin bonding performance.