细菌纤维素-聚乳酸乙醇酸-羟基磷灰石复合支架材料的细胞相容性

目的研究细菌纤维素(bacterial cellulose,BC)-聚乳酸乙醇酸(poly lactic-co-glycolic acid,PLGA)-羟基磷灰石(hydroxyapatite,HA)复合支架与成骨样细胞MG-63的生物相容性。方法通过体外实验,将成骨样细胞MG-63复合于支架材料BC-PLGA-HA和BC-PLGA上,分别为BC-PLGA-HA组和BC-PLGA组,通过扫描电镜、四甲基偶氮唑盐比色法以及碱性磷酸酶检测,观测细胞生长情况。结果细胞接种5 d后,BC-PLGA-HA复合支架材料上的细胞比BC-PLGA支架生长密集,BC-PLGA-HA组、BC-PLGA组以及空白对照组的碱性磷酸酶活性分别为(422.12±0.15)U/L、(289.36±0.21)U/L、(75.34±0.08)U/L,三组差异有统计学意义(F=14.300,P=0.002),BC-PLGA-HA组高于BC-PLGA组,此两组碱性磷酸酶洗活性均高于空白对照组,差异有统计学意义(P<0.05)。结论 BC-PLGA-HA复合支架具有良好的生物相容性,有望用作骨组织工程支架材料。

Compatibility of composite scaffold BC-PL-GA-HA on osteoblasts (MG-63)

Objective To explore the biocompatibility of composite Scaffolds BC-PLGA/HA on osteoblasts（MG-63）.Methods In vitro osteoblasts（MG-63） were seeded on the composite scaffolds BC-PLGA/HA and on the cell culture plate as negative control.Scanning electron microscope（SEM）,3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazoliumbromide（MTT） assay and alkaline phosphatase（ALP） activity test were performed to assess the growth and osteogenic differentiation of osteoblasts（MG-63）.Results Osteoblasts（MG-63） attached on all two scaffolds materials can adhere,grow,differentiate and proliferate.The effect of BC-PLGA-HA scaffolds materials on cell activity was more than that of BC-PLGA scaffolds materials.Conclusion The composite scaffolds BC-PLGA-HA have a good compatibility,indicating that the material has a great potential for application in tissue-engineering.