EGCG和EGCG-3Me对根管牙本质粘接界面稳定性的作用

目的:评价表没食子儿茶素没食子酸酯(EGCG)及其甲基化修饰物(EGCG-3Me)改性粘接剂对根管牙本质粘接界面稳定性的作用.方法:将质量浓度为400 μgml的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2,SB2为对照组.激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能.微拉曼光谱仪检测粘接剂双键转化率.制备纤维桩粘接试件,用于即刻和老化后的微推出实验.结果:改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著.改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P＞0.05).老化后改性粘接剂的微推出粘接强度显著高于SB2(P ＜0.05).结论:EGCG和EGCG-3Me改性的粘接剂均可抑制粪肠球菌增殖并提高树脂-根管牙本质粘接界面稳定性,EGCG-3Me抗菌性能较佳.

The effects of EGCG and EGCG-3Me on the bonding stability of dentin-adhensive to intraradicular dentin

Objective:To evaluate the effect of epigallocatechin-3-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG-3Me) on the anti-bacterial effect and the stability of intraradicular dentin-adhesive interface.Methods:EGCG and EGCG3Me with the concentration of 400 μg/ml were incorporated into Single Bond 2 (SB2) respectively to obtain 2 modified adhesives E-SB2 and E3-SB2.Confocal laser scanning microscopy(CLSM) and ultraviolet spectrophotometry were used to evaluate the anti-bacterial effect of the modified adhesives.Micro-Raman spectrum was used to test the degree of conversion (DC) of the adhesives.Push-out bond strength test was conducted to examine the immediate bond strength and the bond strength after themocycling.Results:E-SB2 and E3-SB2 both showed inhibiting effect on the proliferation of E.faecalis,while E3-SB2 performed stronger inhibiting effect.DC and the immediate push-out bond strength of SB2 were not decreased with the incorporation of EGCG or EGCG-3Me(P ＞ 0.05).E-SB2 and E3-SB2 showed significantly higher push-out bond strengths than that of SB2 (P ＜ 0.05) after themocycling.Conclusion:EGCG and EGCG-3Me modified adhesives have anti-bacterial effect and can enhance the stability of bonding between intraradicular dentin and adhesive,EGCG-3Me may have stronger anti-bacterial effect.