hsa_circ_0003188对人骨髓间充质干细胞成骨分化能力的影响

目的:探讨hsa_circ_0003188对人骨髓间充质干细胞(hBMSCs)成骨分化能力的影响。方法:Human Circular RNA Micro-array Version 2.0芯片检测正常环境与炎性环境(10 ng/mL TNF-α)培养的hBMSCs差异表达circRNAs,并筛选出目的circRNA-hsa_circ_0003188;构建hsa_circ_0003188过表达慢病毒,转染hBMSCs并成骨诱导培养后,通过碱性磷酸酶染色等方法检测hsa_circ_0003188高表达对BMSCs成骨分化能力的影响;利用TargetScan等生物学软件预测hsa_circ_0003188的生物学功能。结果:hsa_circ_0003188在炎性环境hBMSCs中高表达(P<0.05)并抑制其成骨分化;生物学软件预测出hsa_circ_0003188的ceRNA机制。结论:hsa_circ_0003188抑制hBMSCs成骨分化能力,可能成为hBMSCs在骨再生过程中的调控新靶点。

The effects of hsa_circ_0003188 on osteogenic differentiation of bone marrow mesenchymal stem cells in inflammatory environment

Objective:To investigate the effects of hsa_circ_0003188 on osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs).Methods:Human Circular RNA Microarray Version 2.0 microarray was used to detect the differentially expressed circRNAs in hBMSCs cultured in normal and inflammatory environment(10 ng/mL TNF-α)respectively and to screen out the target circRNA-hsa_circ_0003188.After Lent-hsa_circ_0003188 transfection into hBMSCs and osteogenic induction,the effect of high expression of hsa_circ_0003188 on osteogenic differentiation of hBMSCs was detected by alkaline phosphatase staining.TargetScan and other biological softwares were used to predict the biological function of hsa_circ_0003188.Results:hsa_circ_0003188 was highly expressed in hBMSCs in inflammatory environment and Lent-hsa_circ_0003188 inhibited the osteogenic differentiation of hBMSCs.Biology softwares predicted the ceRNA mechanism of hsa_circ_0003188.Conclusion:hsa_circ_0003188 can inhibit the osteogenic differentiation of hBMSCs,which may become a new regulatory target for hBMSCs during bone regeneration.