抗着丝点蛋白B抗体阳性血清的抗核抗体荧光模式及其临床应用价值

目的探讨抗着丝点蛋白B抗体（CENP-B）阳性血清抗核抗体（ANA）荧光模式及其临床应用价值。方法对96例抗CENP-B抗体阳性血清的ANA荧光模式、滴度、分布及临床诊断进行回顾性分析。结果 96例抗CENP-B抗体阳性血清中,单纯性抗CENP-B抗体阳性（＋/-）为46例（47.9%）,＋/-组和＋/＋组的荧光模式比较,差异有统计学意义（P〈0.01）。〈1∶1 000和≥1∶1 000的核型滴度组抗CENP-B抗体阳性强度的差异有统计学意义（P〈0.01）;＋/-组的自身免疫性疾病（AID）患者主要为系统性红斑狼疮、类风湿性关节炎、干燥综合征、系统性硬化症、原发性胆汁性肝硬化和雷诺综合征;非AID患者中,主要多见于脑梗死、贫血和Ⅱ型糖尿病患者。结论抗CENP-B抗体在脑梗死、贫血和Ⅱ型糖尿病患者中的较高表达值得关注。

Fluorescence patterns of anti-nuclear antibody of positive serum of anti-centromere protein B antibody and its clinical application values

Objective To investigate the fluorescence patterns of anti-nuclear antibody（ANA） of positive serum of anti-centro- mere protein B（CENP-B） antibody and its clinical application values.Methods The fluorescence patterns,titers,distribution of ANA of 96 samples of anti-CENP-B antibody-positive serum and clinical diagnosis were retrospectively analyzed.Results Among 96 samples of anti-CENP-B antibody-positive serum,46（47.9%） samples were found simple anti-CENP-B antibody positive（＋/-）,Compared with fluorescence patterns in ＋/- group and ＋/＋ group,difference had statistical significance（P〈0.01）. Difference of anti-CENP-B antibody intensity between1∶1000 and ≥1∶1000 karyotype titer groups showed statistical signifi- cance（P〈0.01）.Systematic lupus erythematosus,rheumatoid arthritis,Sjogren′s syndrome,systemic sclerosis,primary biliary cir- rhosis,and Raynaud′s syndrome were the main disease which were found in patients with autoimmune diseases（AID）in ＋/- group,while cerebral infarction,anemia and typeⅡ diabetes were mainly prevalent in non-AID patients.Conclusion It should be pay attention for the high expression of anti-CENP-B antibody in patients with cerebral infarction,anemia and typeⅡ diabetes.