慢性乙型肝炎患者红细胞天然免疫黏附功能与血清sCR1变化的相关性研究

目的研究乙型肝炎病毒e抗原(HBeAg)阳性慢性乙型肝炎患者红细胞天然免疫黏附功能(RNIAF)与血清可溶性Ⅰ型补体受体(sCR1)的浓度变化,分析二者的相关性,并探讨其临床意义。方法选择48例肝功能正常、血清HBV DNA大于106 U/mL的HBeAg阳性慢性乙型肝炎患者作为肝功能正常组,48例转氨酶高于正常值2倍以上的HBeAg阳性慢性乙型肝炎患者作为肝功能异常组。检测RNIAF,同时采用酶联免疫定量检测试剂盒测定血清sCR1含量。结果肝功能异常组的HBeAg阳性慢性乙型肝炎患者RNIAF较肝功能正常组及健康对照组显著下降,而sCR1含量显著升高,差异有统计学意义(P<0.01)。动态观察持续肝功能异常的HBeAg阳性慢性乙型肝炎患者RNIAF与血清sCR1含量变化,显示二者呈明显负相关(r=-0.91)。HBeAg阳性慢性乙型肝炎患者血清sCR1含量与凝血酶原活动度也呈明显负相关(r=-0.87)。结论 HBeAg阳性慢性乙型肝炎患者RNIAF与血清sCR1的含量变化与肝脏功能损伤严重程度密切相关,可作为临床判断肝病患者病情严重程度及预后的参考指标。

Relationship between the red cell nature immune adhesion function and the concentrations of soluble complement receptor type 1 in patients with e antigen positive

Objective To study the changes and significance of red cell nature immune adhesion function（RNIAF） and the concentrations of soluble complement receptor type 1（sCR1） in patients with e antigen positive.Methods 48 cases of e antigen positive with normal hepatic function and HBV DNA much more 106 IU/mL in serum have been used in this study.Equal cases of ALT twice over normal level with or without jaundice have been used as abnormal hepatic function groups.The function of red cells nature immune-adhering tumor cell assay was performed and the concentration of sCR1 was detected by ELISA.Results The RNIAF of patients e antigen positive with abnormal hepatic function was obviously lower than that of patients with normal hepatic function and healthy individuals（P0.01）.But the concentration of sCR1 was significantly higher（P0.01）.The RNIAF was evidently related with the changes of sCR1 in patients of e antigen positive with continually abnormal hepatic function（r=-0.91）.The sCR1 concentrations of patients with e antigen positive were negatively related with PTA（r=-0.87）.Conclusion There is a good relationship between RNIAF,serum sCR1 and the degree of liver damage.The two parameter may be as an important marker for judging the degree and prognoses of liver diseases.