乙型肝炎病毒外膜大蛋白检测的临床意义

目的探讨乙型肝炎患者血清中乙型肝炎病毒外膜大蛋白（HBVLP）检测的临床意义。方法采用酶联免疫吸附试验检测HBVLP；采用化学发光法检测HBV标志物；采用荧光定量聚合酶链反应检测HBVDNA。结果317例HBsAg阳性标本中HBVDNA与HBVLP通过Kappa检验得出的结论一致性较好（P〈0．01，Kappa=0．85）。221例HBeAg阴性患者标本中HBVLP阳性检出率高于HBVDNA阳性检出率，分别为53．39％和47．06％，采用Y。检验，差异有统计学意义（P〈0．05）。196例HBVDNA阳性标本中HBVDNA拷贝数对数值与HBVLPA值之间有良好的相关性（r=0．982）。结论HBVLP水平能反映乙型肝炎患者体内HBV复制程度，而且枪测操作简便．具有较好的临床实用价值。

Clinical significance of HBV large protein detection in patients with chronic hepatitis B

Objective To investigate the clinical significance of hepatitis B virus large protein（HBV LP） in pa- tients with chronic hepatitis B（CHB）. Methods HBV LP level was analyzed by enzyme linked immunosorbent as- say,serum marker of HBV were detected by chemo-luminescence method. ,and HBV DNA was quantitatively detec- ted by real-time quantitative polymerase chain reactioin. Results No significant difference of the detectable rate be- tween HBV DNA and HBV LP was found in 317 cases of HBsAg positive patients （P〈0.01,Kappa=0.85）. The positive rates of HBV DNA and HBV LP in 221 cases of HBeAg negative patients were 47.06% and 53.39% respectively,with statistical difference（P~0.05）. The absorbance（A value） of serum HBV LP was positively correla- ted with HBV DNA copies（r= 0. 982）. Conclusion HBV LP could be a reliable serological marker, reflecting the replication of HBV,with important clinical value.