选择Jaffe法消除胆红素对肌酐测定的干扰

目的通过调整全自动生化仪测试参数,合理选择比色时间,以消除高胆红素对Jaffe法测定肌酐的干扰。方法使用Jaffe速率法测定血清肌酐,不加入任何化学物品,只通过调整迈瑞BS400全自动生化分析仪的比色周期来消除高胆红素对肌酐测定的干扰。结果试验组(高胆红素组)和对照组(低胆红素组)在44～49比色周期之间读取吸光度变化,可消除高胆红素对肌酐测定的干扰(0.01P0.05)。结论根据仪器特点,合理调整参数可有效排除高胆红素对肌酐测定的干扰,取得与低胆红素含量条件下相同的结果。

Study of the feasibility of elimination of the interference of high concentration bilirubin to creatinine test by Jaffe kinetic assay method

Objective To eliminate interference of high concentration bilirubin in creatinine test with Jaffe kinetic assay method by adjusting the test parameters and reasonable choose of the colorimetric time of automatic biochemical analyzer. Methods Jaffe kinetic assay method was employed for serum creatinine detection. Only by adjusting colorimetric cycle of BS-400 automatic biochemical analyzer and without adding any chemicals,we can eliminate the interference of high concentration bilirubin involved in the creatinine detection.Results Obtaining the variety of absorbance value during 44 to 49 colorimetric cycle between the test groups （high concentration bilirubin groups） and control groups （low concentration bilirubin groups） might eliminate the interference of high concentration bilirubin for creatinine detection（0.01P0.05）. Conclusion According to the feature of this instrument,reasonable adjustment of parameters could exclude the interference of high concentration bilirubin in the creatinine determination effectively,and achieve the same test results with low bilirubin level.