前尿激酶基因转移对血小板沉积兔颈动脉内膜的抑制作用

观察由腺病毒载体介导的前尿激基因导入家兔颈动脉后的表达和对血小板在损伤动脉内膜沉积的影响。方法将ｐｒｏＵＫ基因腺病毒载体局部注射入家兔右颈总动脉腔内用野生型腺病毒（Ａｄ）局部注射入左颈总动腔内作为自身对照。用免疫组织化学染色观察ｐｒｏＵＫ基因因在血管内膜的表达;家兔血小板用＾１１１Ｉｎ标记并回输体内后,用电刺激务转基因血管内膜,定量观察＾１１１Ｉｎ标民血小板在损伤血管内膜沉 量,血管切片ＨＥ染色

The inhibition of prourokinase gene transfer on deposition of platelets on rabbit carotid artery intima

OBJECTIVE: To observe the expression of pro-urokinase (proUK) gene in rabbit carotid artery transfected with replication-deficient adenovirus vector containing proUK gene (Ad/prouk) and the deposition of platelet on the same injured vessel. METHODS: Ad/proUK (Ad/proUK, 3 x 10(10) pfu/ml) was injected into the right carotid artery locally. Wild type adenovirus (Ad) was locally injected into the left carotid artery as self-control. The expression of proUK gene was investigated by immunohistochemistry assay. After injury of the gene-transfected vessel by electric stimulation, the deposition of (111) In-labeled platelet was quantitatively observed. The thrombosis was observed with HE-stained vessel section. RESULTS: There were lots of proUK granules in the endothelium of Ad/proUK gene-transfected vessel. The differences in (111) In-platelets deposition per gram dry weight vessel segment were significant between Ad/proUK and Ad transfected control vessels (4.60 +/- 0.93) x 10(7)/g vs control (27.95 +/- 4.93) x 10(7)/g, P < 0.01)]. The HE-stained vessel section showed that there were only small thrombi in proUK gene-transfected vessel but massive thrombi almost blocked up the whole vessel in control vessel. CONCLUSION: The proUK gene-transfected vessel can obviously inhibit (111) In-labeled platelet deposition on injured vessel and thus thrombosis.