不同压力高压氧对脑出血大鼠Bcl-2/Bax比值的影响

目的:观察不同压力高压氧(hyperbaric oxygen,HBO)对脑出血(intracerebral hemorrhage,ICH)大鼠出血灶周围组织Bcl-2/Bax比值的影响。方法:应用胶原酶诱导法建立大鼠脑出血模型,将90只雄性脑出血SD大鼠随机分为对照组、高压氧治疗组:1.0ATA组、1.8ATA组、2.0ATA组、2.2ATA组,每组18只,按不同治疗压力于造模后24h进行治疗,1次/d,各组分别于造模成功后第1、3、5天断头取脑,每个时间点各6只。免疫组织化学染色法测定Bcl-2与Bax的表达,对Bcl-2/Bax比值进行统计分析。结果:Bcl-2/Bax比值:第1天时,高压氧治疗组较对照组升高,差异有显著性意义(P0.05),1.8ATA组、2.0ATA组、2.2ATA组较1.0ATA组升高,差异有显著性意义(P0.05),2.2ATA组、2.0ATA组、1.8ATA组依次升高,1.8ATA组、2.0ATA组与2.2ATA组比较差异有显著性意义(P0.05),1.8ATA组与2.0ATA组比较差异无显著性意义(P0.05);第3天时,高压氧治疗组较对照组升高,1.0ATA组与对照组比较差异无显著性意义(P0.05),1.8ATA组、2.0ATA组、2.2ATA组与对照组比较差异有显著性意义(P0.05),2.2ATA组、2.0ATA组、1.8ATA组依次升高,各组之间比较差异均有显著性意义(P0.05);第5天时,高压氧治疗组较对照组升高,差异均有显著性意义(P0.05),2.0ATA组、2.2ATA组低于1.0ATA组,差异无显著性意义(P0.05),1.8ATA组高于1.0ATA组、2.0ATA组、2.2ATA组,差异有显著性意义(P0.05)。结论:HBO治疗可提高出血灶周围脑组织Bcl-2/Bax的比值,抑制出血灶周围组织细胞凋亡,且1.8ATA在提高Bcl-2/Bax的比值上较优。

Effects of different atmosphere absolute hyperbaric oxygen on the ratio of Bcl-2/Bax in rats with intracerebral hemorrhage

Abstract Objective:To study the effect of different atmosphere absolute(ATA) hyperbaric oxygen(HBO) on the ratio of Bcl-2/Bax in rats with intracerebral hemorrhage(ICH). Method:The collagenase-induced method was used to set up the rat models of ACH.A total of 90 male SD rats with experimental intracerebral hemorrhage were randomly divided as ICH control group, CH with HBO therapy group, 1.0ATA group,?1.8ATA group, 2.0ATA group, 2.2ATA group,with 18 rats in each group.The oxygen treatment was administered by using 90% oxygen at different ATA, 24 hours after ICH, once daily. Rats were sacrificed by decollation at the time points of 1d, 3d, 5d. Each time point had 6 rats.The expressions of Bcl-2 and Bax were detected by immunohistochemical staining. Statistical analysis for the ratio of Bcl-2/Bax was performed. Result: The ratio of Bcl-2/Bax increased from 1d to 5d gradually for the control ICH group. The ATA groups increased gradually also but significantly more than the control group at any time point. The most increasement happened in 1.8 ATA group. The 2.0 and 2.2 ATA groups increased? less and less gradually.The least is the 1.0 ATA group. Conclusion:HBO therapy can increase the ratio of Bcl-2/Bax and play a role of inhibition of nerve cell apoptosis and neuroprotection.1.8ATA was superior to others.