类风湿关节炎患者血清IL-35，MMP-3，Gal-1水平及其与免疫功能指标的相关性研究

目的　探讨类风湿关节炎患者血清白细胞介素35（IL-35），基质金属蛋白酶3（MMP-3），半乳糖凝集素1（Gal-1）水平变化及与免疫功能指标的相关性研究。方法　选择2018 年2 月～ 2019 年2 月陕西省榆林市第一医院接诊的80 例类风湿关节炎患者为观察组，并选择同期体检的健康人70 例为对照组，分析血清IL-35，MMP-3，Gal-1 和免疫球蛋白（Ig）A，IgG 和IgM 水平变化情况，采用Spearman 相关系数分析血清IL-35，MMP-3 和Gal-1 与免疫功能指标之间的相关性。结果　观察组血清IL-35，MMP-3 和Gal-1 水平分别为147.82±37.95pg/ml，81.36±37.34ng/ml和49.38±13.58μg/L， 对照组血清IL-35，MMP-3 和Gal-1 水平分别为46.35±15.54pg/ml，30.16±6.52ng/ml 和37.25±8.21μg/L，观察组上述指标水平均显著高于对照组，差异均有统计学意义（t=20.844，11.318，6.504，均P＜ 0.05）；活动组血清IL-35，MMP-3 和Gal-1 水平分别为162.02±38.16 pg/ml，90.52±36.75ng/ml 和53.11±14.02μg/L，缓解组血清IL-35，MMP-3 和Gal-1 水平分别为129.56±35.89pg/ml，69.58±31.52ng/ml 和44.59±13.21μg/L，活动组上述指标水平均显著高于缓解组，差异有统计学意义（t=3.873，2.688，2.765，均P ＜ 0.05）；观察组IgA，IgG 和IgM 水平分别为2.86±0.53g/L，16.85±4.71g/L 和1.78±0.82g/L，对照组IgA，IgG 和IgM 水平分别为1.57±0.31g/L，13.26±3.56g/L 和1.21±0.51g/L。观察组上述指标水平均显著高于对照组，差异有统计学意义（t=17.861，5.206，5.026，均P ＜ 0.05）；活动组IgA，IgG 和IgM 水平分别为3.11±0.56g/L，18.07±4.76g/L 和1.97±0.85g/L，缓解组IgA，IgG 和IgM 水平分别为2.54±0.49g/L，15.28±4.18g/L 和1.54±0.75g/L，活动组上述指标水平均显著高于缓解组，差异有统计学意义（t=4.766，2.741，2.362，均P ＜ 0.05）；将血清IL-35，MMP-3 和Gal-1 作为因变量，将免疫功能指标水平分别作为自变量，在相关性分析结果中显示，血清IL-35，MMP-3，Gal-1 与IgA，IgG，IgM 之间均呈正相关（r=0.223~0.618，均P ＜ 0.05）。结论　在类风湿关节炎患者中血清IL-35，MMP-3 和Gal-1 的表达与免疫功能指标之间联系紧密。

Study on the Changes of Serum IL-35, MMP-3, Gal-1 Levels and Their Correlation with Immune Function Indexes in Patients with Rheumatoid Arthritis

Objective　To investigate the changes of serum levels of Interleukin 35 (IL-35), matrix metalloproteinase 3 (MMP-3) and galactosin 1 (Gal-1) in patients with rheumatoid arthritis and their correlation with immune function indicators. Methods　80 patients with rheumatoid arthritis who were admitted to the First Hospital of Yulin City from February 2018 to February 2019 were selected as the observation group.and 70 healthy people in the same period of physical examination in the hospital were selected as the control group.Their serum IL-35,MMP-3,Gal-1 and immunoglobulin (Ig)A, IgG and IgM levels were analyzed.The correlation between serum IL-35, MMP-3, Gal-1 and immune function was analyzed by Spearman correlation coefficient. Results　The levels of serum IL-35, MMP-3 and Gal-1 in the observation group were 147.82±37.95pg/ml, 81.36±37.34 ng/ml and 49.38±13.58μg/L, respectively. The levels of serum IL-35，MMP-3 and in the control grouup were 46.35±15.54 pg/ ml, 30.16±6.52 ng/ml and 37.25±8.21μg/L, respectively. The levels of above indexes in the observation group were significantly higher than those in the control group, the differences were statistically significant (t=20.844, 11.318, 6.504, all P < 0.05). The levels of serum IL-35, MMP-3 and Gal-1 in the active group were 162.02±38.16 pg/ml, 90.52±36.75ng/ml and 53.11±14.02μg/L, respectively. The levels of serum IL-35, MMP-3 and Gal-1 in the control group were 129.56±35.89 pg/ ml, 69.58±31.52 ng/ ml and 44.59±13.21μg/L, respectively. The levels of the above indexes in the activity group were significantly higher than those in the remission group, the differences were statistially significant (t=3.873, 2.688, 2.765, all P < 0.05). The levels of IgA, IgG and IgM in the observation group were 2.86±0.53 g/L, 16.85±4.71 g/L and 1.78±0.82 g/L, respectively. The levels of IgA, IgG and IgM in the control group were 1.57±0.31g/L, 13.26±3.56 g/L and 1.21±0.51 g/L，respectively, and the above indexes in the observation group were significantly higher than those in the control group, the differences were statistically significant (t=17.861, 5.206, 5.026, all P < 0.05).The levels of IgA, IgG and IgM in the active group were 3.11±0.56 g/L, 18.07±4.76 g/L and 1.97±0.85 g/L, respectively. The levels of IgA, IgG and IgM in the remission group were 2.54±0.49 g/L, 15.28±4.18 g/L and 1.54±0.75 g/L，respectively，and the above indexes in the activity group were significantly higher than those in the remission group, the differences were statistically significant (t=4.766, 2.741, 2.362, all P < 0.05). Serum IL-35, MMP-3 and Gal-1 were taken as dependent variables, and the level of immune function indexes were taken as independent variables respectively. Correlation analysis results showed that serum IL-35, MMP-3 and Gal-1 were positively correlated with IgA, IgG and IgM (r=0.223~0.618, all P < 0.05). Conclusion　The expression of serum IL-35,MMP-3 and Gal-1 in rheumatoid arthritis patients were closely related to immune function indexes.