针刺损伤免椎间盘退变模型组织病理变化及软骨样细胞的迁移规律

背景：兔椎间盘退变模型间盘退变表现为随时间进展脊索细胞将被软骨样细胞逐渐替代,但兔针刺纤维环间盘退变模型中软骨样细胞的来源和移行规律尚不明确。目的：观察针刺兔纤维环间盘退变模型椎间盘病理变化过程,并初步探讨软骨样细胞来源及移行规律。方法：将24只新西兰大白兔随机分为手术组与假手术组。手术组使用16G穿刺针针刺L2/L3、L3/L4、L4/L5及L5/L6椎间盘纤维环,假手术组暴露至相同椎间盘前方后冲洗闭合伤口。结果与结论：针刺损伤椎间盘退变过程中的软骨样细胞来源于终板。在髓核与上下终板交界处,软骨细胞脱离终板成串向髓核中心迁移;在髓核与内层纤维环交界处,软骨细胞沿纤维走行迁移并随之向皱缩的髓核边缘迁移。椎间盘退变过程中非钙化层逐渐变薄,非钙化层/钙化层比值逐渐降低。

Histopathological changes and rule of migration associated with chondrocytes in acupuncture-induced intervertebral disc degeneration model in rabbits

BACKGROUND：The notochordal cells will be replaced by chondrocytes along with the aggravation of degenerative intervertebral disc,but the origin and migration rule of the chondrocytes of the model established by puncturing the annulus fibrosus are still unclear.OBJECTIVE：To observe the histopathological process of degenerative intervertebral disc model established by puncturing the annulus fibrosus,and to explore the origin and migration rule of the chondrocytes.METHODS：Twenty-four New Zealand white rabbits were divided into operation group and sham operation group.In the operation group,lumbar discs of L2-3,L3-4,L4-5 and L5-6 of each rabbit were punctured by 16 gauge needle.In the sham operation group,the anterior surfaces of the lumbar discs were exposed without puncture.RESULTS AND CONCLUSION：The chondrocytes of the nucleus pulposus originated and migrated from the cartilage endplate.At the junction of the nucleus pulposus and the cartilage endplate,the chondrocytes departed from the cartilage endplate to the nucleus pulposus vertically.At the junction of the inner annulus fibrosus and the cartilage endplate,the chondrocytes migrated along the collagen fibers from the cartilage endplate to the shrinked margin of the nucleus pulposus.The non-calcified layer thinned gradually,and the non-calcified layer/calcified layer ratio decreased.