血必净注射液对脓毒症大鼠血栓调节蛋白及内皮蛋白C受体基因表达的影响

目的探讨血必净注射液对脓毒症大鼠血栓调节蛋白（TM）及内皮蛋白C受体（EPCR）基因表达的影响。方法采用盲肠结扎穿孔术（CLP）制备脓毒症模型。将96只健康Wistar大鼠按随机数字表法分为正常对照组、假手术组、模型组和血必净治疗组,后两组又按处死时间分为术后2、8、24、48和72h亚组,每组8只。留取肝、肺组织,分别检查各组动物组织TM和EPCR的mRNA表达。结果正常对照组和假手术组肝、肺组织TM和EPCR的mRNA有一定表达。CLP后2h肝、肺组织TM和EPCR的mRNA表达无明显变化（P均〉0.05）,8～48h肝、肺组织TM和EPCR的mRNA表达均有不同程度的增强（P均〈0.01）,至伤后72h基本恢复到术前水平（P均〉0.05）;与模型组比较,血必净治疗组CLP后8h和24h组织TM和EPCR的mRNA表达均有不同程度下降,48h和72h的基因表达有不同程度提高。结论血必净注射液可以从基因水平影响脓毒症动物组织TM及EPCR的基因表达。

Effects of Xuebijing injection (血必净注射液) on thrombomodulin and endothelial cell protein C receptor in septic rats

OBJECTIVE: To investigate effects of the integrated traditional Chinese medicine Xuebijing injection on thrombomodulin (TM) and endothelial cell protein C receptor (EPCR) in septic rats. METHODS: Ninety-six healthy Wistar rats were randomly divided into four groups: control group, sham operation group, cecal ligation and puncture (CLP) model group, and Xuebijing-treated group. Sepsis was reproduced by CLP. The two latter groups were divided into five subgroups of 2, 8, 24, 48 and 72-hour with 8 rats in each subgroup. Tissue samples from liver and lung were collected to determine tissue TM and EPCR mRNA expression. RESULTS: TM and EPCR mRNA expressions were observed in liver and lung in control group and sham operation group, while with no significant differences at 2 hours post-CLP (both P>0.05). TM and EPCR gene expression levels in tissues were significantly increased to certain extent at 8-48 hours (all P<0.01), and were dramatically decreased following Xuebijing injection at 72 hours post-CLP (both P>0.05). Also, treatment with Xuebijing injection markedly decreased TM and EPCR mRNA levels to certain extents at 8 and 24 hours, and markedly increased at 48 and 72 hours compared with those of model group. CONCLUSION: These data suggest that Xuebijing injection could raise TM and EPCR mRNA expression, thereby it might be effective in prevention of development of severe sepsis.