磺基水杨酸检测尿液和脑脊液蛋白自动比浊法的建立

目的对磺基水杨酸比浊法进行改良,并对其测定尿液和脑脊液蛋白的自动化分析进行评价。方法应用改良磺基水杨酸仪器比浊法(简称仪器法),在全自动生化分析仪上建立测定参数,评价其方法的精密度、线性范围、回收率,并与手工磺基水杨酸比浊法(简称手工法)进行比较。结果批内和批间变异系数(CV)分别为1.2%～1.5%和2.1%～3.5%。线性范围为0.037～2.38 g/L。回收率为85.6%～109.2%。与手工法比较,Y仪器=0.980 4X手工+0.010 8,相关系数(r)=0.982。结论改良的磺基水杨酸比浊法测定微量总蛋白具有较高的精密度和准确度,适合于全自动生化分析仪。

Establishment of a method for detecting urine and cerebro-spinal fluid proteins by sulfosalicylic acid turbidimetry using automatic biochemical analyzer

Objective To evaluate and improve the method for detecting urine and cerebro-spinal fluid proteins by sulfosalicylic acid turbidimetry using automatic biochemical analyzer.Methods The improved sulfosalicylic acid turbidimetry using automatic biochemical analyzer（I-SS assay） and its parameters were established.The precision,linear range and recovery rate were evaluated and compared with the traditional sulfosalicylic acid turbidimetry（T-SS assay）.Results The within-run and between-rum coefficients of variation（CV） were 1.2%-1.5% and 2.1%-3.5%.The linear range was 0.037-2.38 g/L.The recovery rate was 85.6%-109.2%.The correlation with the T-SS assay was Y I-SS = 0.980 4X T-SS ＋0.010 8,and the correlation coefficient（r） was 0.982.Conclusions The I-SS assay is sensitive and accurate,and it is suitable to automatic biochemical analyzer.