电针对炎性痛大鼠背根节BSI-B4表达的调节观察

目的观察炎性痛条件下西非单叶豆同工凝集素(BSI-B4,可以选择性标记参与痛觉信息传递的C纤维及其终末)结合位点的变化情况以及电针对其调节。方法采用大鼠单侧佐剂性关节炎性痛模型,采用免疫荧光组织化学技术观察了致炎后不同时间段(分别取第3、7d)背根节BSI-B4结合位点的表达情况,以及在针刺(电针环跳穴、阳陵泉穴)条件下该指标的变化情况。结果注射后第3、7d各组大鼠背根节的BSI-B4结合位点表达关系比较如下:炎性痛组(包括3、7d组)>电针组(包括3、7d组)>生理盐水组(包括3、7d组)(P<0.01)。且炎性痛组大鼠BSI-B4表达还表现为3d组>7d组(P<0.05)。结论电针可以下调炎性痛大鼠背根节BSI-B4结合位点表达的增多,这可能是电针镇痛的机理之一,即通过减缓炎性痛大鼠C伤害性感受器的持久发放达到治疗炎性痛的目的。

Electroacupuncture Modulation on BSI-B4 Binding Sites Expression of Inflammatory Rats

Objective To observe the changes of Banderaea simplicifolia Isolectin B4(BSI-B4) binding sites expression in the dorsal root ganglion(DRG) following inflammatory pain and elucidate the influence of electroacupuncture(EA) to it.Methods In this study,we applied the complete Freund's adjuvant(CFA)-induced inflammatory pain model.In addition,BSI-B4 binding sites were detected in DRG by using immunofluresence histochemistry following CFA-induced inflammation and EA treatment.Results At both 3~(rd) day and 7~(th) day following injection,the mean immunoreactive area and optical density of BSI-B4 labeled products,respectively,all showed that: inflammatory group>EA treatment group>control group(all P<0.01).And in inflammatory group,the expression of N BSI-B4 also showed that: 3~(rd)day group>7~(th) day group(P<0.05).Conclusion The above data indicated that EA could down-regulate the BSI-B4 binding sites expression in DRG.It maybe one of the mechanism of EA analgesia that was probably to weaken the discharge of unmyelinated nociceptive afferent terminals which contributed significantly to the hyperalgesia.