脓毒症大鼠肠道肠三叶因子mRNA表达的变化

目的：探讨脓毒症大鼠肠道肠三叶因子（trefoil factor family,TFF3）mRNA表达的变化。方法：32只SD大鼠随机分为对照组（n=8）和脓毒症组（n=24）。采用盲肠结扎穿孔术（cecal ligation and puncture,CLP）制作大鼠脓毒症模型。于模型建立后3h,6h及12h（n=8）取回肠黏膜,以RT-PCR法检测TFF3 mRNA的表达。结果：脓毒症模型建立3h始TFF3 mRNA表达即显著下降（P〈0.01）,随着时间延长表达进一步下降。结论：脓毒症大鼠肠道TFF3 mRNA表达明显下降,可能是脓毒症肠屏障功能障碍的机制之一并延缓肠道黏膜修复。

Changes of Expression of Intestinal Trefoil Factor Family mRNA in The Rat Model of Sepsis

Objective:To investigate the changes of expression of intestinal trefoil factor family mRNA in the rat model of sepsis. Method: Thirty-two Sprague-Dawley rats were randomly divided into control(n=8) and sepsis (n=24). Sepsis was induced by cecal ligation and puncture (CLP). At 3 h, 6 h and 12 h (n=8) after CLP, the rats were killed and some ileum were removed and the expression of TFF3 mRNA was detected with RT-PCR. Results: The expression of TFF3 mRNA in sepsis group significantly decreased compared to control at 3h after CLP and decreased (P<0.01) and further decreased along with time. Conclusions: The expression of intestinal TFF3 mRNA significantly decreased in sepsis, which would be one of mechanisms in dysfunction of intestine barrier in sepsis and could delay intestinal mucosal recovery.