偏头痛大鼠硬脑膜及三叉神经节上TRPV1的表达变化

目的:观察TRPV1在偏头痛大鼠硬脑膜及三叉神经节上的表达变化。方法:取健康成年雄性SD大鼠60只,按随机数字法分为对照组(control组)、实验复方致炎剂(inflammation soup,IS)1 d组(IS 1 d组)、实验IS 3 d组(IS 3 d组)和实验IS 6 d组(IS 6 d组)。在雄性SD大鼠硬脑膜上埋置PE-10管,实验组分别给予1 d、3 d、6 d IS 20μl/d及对照组给予6天等量生理盐水。采用WesternBlot、免疫荧光法和Real-Time PCR法检测TRPV1在硬脑膜及三叉神经节上的表达情况。结果:对照组和实验组大鼠硬脑膜及三叉神经节上均可见TRPV1表达;与对照组相比,实验组硬脑膜、三叉神经节上TRPV1蛋白表达量、阳性细胞数及mRNA明显升高,以实验IS 6 d组最高(P<0.05)。结论:TRPV1在偏头痛大鼠硬脑膜及三叉神经节上的表达增加。TRPV1表达上调可能是偏头痛外周敏化的重要机制之一。

THE EXPRESSION OF TRPV1 IN DURA mater AND TRIGEminAL GANGLIa OF MIGRAI-NE RATS

Objective:To observe the expression of TRPV1 in dura mater and trigeminal ganglia of migraine rats.Methods:Sixty adult male Sprague Dawley rats were randomly divided into 4 groups:control group one day inflammation soup infusion group(IS 1 d group),three day is infusion group(IS 3 d group),six day is infusion group(IS 6 d group).Dural inflammation soup(IS) was injected through PE-10 tubing on the dura in male rats.Western blot analysis,immunofluorescence technique and real-time PCR were used to observe the expression of TRPV1 in dura mater and trigeminal ganglia.Results:TRPV1 was expressed in each group and the TRPV1 protein expression,the positive cell number,and the mRNA of the experimental group were significantly higher than those of the control group,the IS 6 d group was the highest(P < 0.05).Conclusion:TRPV1 expression is increased in dura mater and trigeminal ganglia of migraine rats.The increase of TRPV1 expression may be one of the important mechanisms of peripheral sensitization.