CD90和CD44在人肝癌组织中的表达及临床意义

目的:研究肝癌干细胞(liver cancer stem cells,LCSCs)表面标志物:CD90和CD44在人肝癌组织中的表达情况及其与肝癌复发的关系。方法:收集20例已经随访1年以上的原发性肝细胞肝癌患者经手术切除的组织标本。20例患者中,术后1年内复发和无复发各10例。标本用石蜡包埋,以1μm的厚度连续切3片。1片做HE染色,用于病理诊断。另外2片则用作免疫组织化学检测,以观察CD90和CD44的表达情况,在显微镜下观察、摄片,检测各标志物在肝癌组织的阳性率,并比较它们在复发病例和无复发病例间的表达差异。结果:CD90和CD44在不同个体的肝癌组织中表达有差异,两者的阳性率均为0.13%～4.10%。CD90和CD44的表达与肝癌的预后有一定的关系,CD90在复发组的表达显著高于无复发组,且复发组CD90或CD44阳性的LCSCs的临近血管率均显著高于无复发组。结论:肝癌患者肿瘤组织中的LCSCs有明显异质性,且它们可同时表达多种肿瘤干细胞标志物。LCSCs标志物的阳性率及其与血管的关系和肝癌复发有一定关系。

Expression and Clinical Significance of CD90 and CD44 in Human Hepatocarcinoma Tissues

To study the expression of CD90 and CD44 in human hepatocelluar carcinoma （HCC） tissues, and to ex- plore the relationship between their expression and recurrence of HCC. Methods： We collected tissue samples of 20 HCC pa- tients, who had been followed up for more than one year, including 10 cases with recurrence and 10 cases without recurrence. Specimens were embedded in paraffin, and then 3 pieces were cut from each specimen in the thickness of 1μm. The first piece was routinely HE stained for pathological diagnosis. The other two consecutive pieces were used to detect CDg0 and CD44 by immunohisto- chemistry. The expression of CD90 and CD44 in recurrence group and recurrence-free group was compared. Results： The expression of CD90 and CD44 in human HCC tissues was from 0.13% to 4. 10%. The expression of CD90 and CD44 had a relationship with HCC recurrence and prognosis. The expression of CDg0 in the recurrent group was significantly higher than that in the recurrent-free group. Most of CD90 or CD44 positive cells were closed to vessels in recurrence group. Conclusions： Liver cancer stem cells exist in HCC tumor tissues with significant heterogeneity, and they express a variety of cancer stem cell markers such as CDg0 and CD44. The ex- pression of liver cancer stem cell markers is correlated with recurrence of HCC.