针刀疗法对缺血缺氧性幼鼠脑组织CX32及Nestin蛋白表达的影响

目的:目的:观察针刀刺激对幼鼠缺血缺氧性脑损伤的影响。方法:新生7日龄SD幼鼠28只,随机分为手术组20只及假手术组8只。手术组幼鼠复制为缺血缺氧性脑损伤模型,共成功16只,再随机人为手术组A和手术组B各8只。手术组A于造模后2、9及16d时参照《实验针灸学》取百会、大椎、曲池、内关、环跳、阳陵泉及三阴交穴分别交替给予针刀治疗1次。造模第17天时通过悬吊试验观察各组随意运动功能,免疫组化观察脑组织缝隙连接蛋白(CX32)及巢蛋白(Nestin)的表达。结果:悬吊试验中,手术组A平均悬吊时间评分与假手术组比较差异不明显;手术组B则明显低于假手术组(P0.05)。CX32表达,手术组B明显低于手术组A及假手术组(P0.05),手术组A与假手术组比较差异无统计学意义。Nestin表达,手术组A与假手术组及手术组B比较均明显升高(P0.01)。结论:针刀治疗缺血缺氧性脑损伤可改善随意运动功能障碍,启动内源性神经干细胞的增殖,提高损伤后脑组织CX32的表达。

Effects of Acupotomy Therapy on CX32 and Nestin Expression in Hypoxia-ischemic Brain Injury of Neonatal Rats

Objective:To observe the effects of acupotomy therapy on hypoxia-ischemic brain damage (HIBD) in neonatal rats. Methods:Twenty-eight 7-day-old SD rats were randomly divided into 3 groups:sham operation group,HIBD group and acupotomy group. After the models were made successfully,acupotomy therapy was given since the second day,and then once every 7 days. In the 24-day-old rats the voluntary motor function was observed in hanging test,and their brains were removed for detection of gap junction connexin32 (CX...