无水乙醇和聚桂醇增强聚焦超声消融兔肝脏实验研究

目的探讨聚焦超声（FUS）的超声效应对无水乙醇（EA）、聚桂醇（LM）消融兔肝的协同作用。 方法选用健康新西兰大白兔40只，随机分为5组：EA组、LM组、FUS＋EA组、FUS＋LM组，FUS组，每组8只。FUS焦点的平均声强（I_SPTA）30.0 W/cm²，工作占空比50%。按分组对兔正常肝右叶根部给予消融处理，48 h后行超声造影分析并获取肝脏组织进行病理检查，测量消融灶大小和体积。采用Kruskal-Walis H秩和检验比较各组实验兔肝脏消融体积，进一步采用组间两两比较。 结果各组的消融效果，差异有统计学意义（H=31.05，P<0.001）。FUS＋EA组、FUS＋LM组、EA组及LM组实现了对正常兔肝不同程度的消融，而FUS组没有产生任何微血管及病理损伤。进一步组间两两比较，FUS＋EA组肝脏消融体积显著，差异有统计学意义（Z=-2.80、-2.82、2.84、5.57，P均<0.05）；FUS＋LM组、EA组、LM组和FUS组组间，差异无统计学意义（P>0.05）。 结论FUS激励EA能够产生显著的协同效应，有效增大EA对兔肝的消融体积；FUS激励聚桂醇无协同效应。

Focused ultrasound combined with absolute ethanol or lauromacrogol for ablation of rabbit liver:an experimental study

Objective To study the synergistic effect of focused ultrasound(FUS)combined with absolute ethanol(EA)and lauromacrogol(LM)in the ablation of rabbit liver.Methods Forty healthy New Zealand white rabbits were randomly divided into five groups:EA group,LM group,FUS plus EA(FUS+EA)group,FUS plus LM(FUS+LM)group,and FUS alone group.The right lobe of the normal liver of rabbits was then ablated.Forty-eight hours later,contrast-enhanced ultrasound(CEUS)was performed on the ablated lobes,and the size,shape,and boundary of the ablation lesions were observed.Liver tissue was obtained after angiography,and the volume of liver ablation was measured by drainage method.Kruskal-Walis H rank sum test was used to compare the ablation volumes among the experimental rabbits in each group,and between the groups.Results The ablation effects among different groups were statistically different(H=31.05,P<0.001).The FUS+EA group,FUS+LM group,EA group,and LM group achieved ablation of the normal rabbit liver to varying degrees,while the FUS group did not produce any microvascular or pathological damage.Further pairwise comparison showed that the FUS+EA group had the most significant liver ablation(Z=-2.80,-2.82,2.84,and 5.57,respectively,P<0.05).There was no significant difference between the FUS+LM group,EA group, LM group, and FUS group (P > 0.05). Conclusion FUS plus EA can produce significant synergisticeffects and effectively enhance the ablation effect of EA alone on the rabbit liver in vivo. No synergistic effect isgenerated by FUS plus lauromacrogol.