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| 化疗药物对体外培养同步化MCF-7细胞存活和凋亡的影响 | |
| 引用本文： | 刘亚辉,刘波,谭岩.化疗药物对体外培养同步化MCF-7细胞存活和凋亡的影响[J].中国实验诊断学,2007,11(3):384-387. |
| 作者姓名： | 刘亚辉 刘波 谭岩 |
| 作者单位： | 1. 吉林大学第一临床学院,普通外科,吉林,长春,130021 2. 吉林大学第一临床学院,药剂科,吉林,长春,130021 3. 吉林大学第一临床学院,中心实验室,吉林,长春,130021 |
| 摘 要： | 目的探讨不同化疗药物在体外培养的经TAM同步化MCF-7细胞存活率和凋亡的影响。方法以体外培养雌激素受体阳性细胞MCF-7为研究对象，采用MTr法和流式细胞术法检测MCF-7细胞存活率和细胞凋亡。结果体外培养的MCF-7细胞的倍增时间是33h，TAM可使MCF-7同步化于G0／G1期，5．Fu、DDP、ADM在中低剂量时可使同步化的MCF-7细胞存活率降低，与不同步化组比较差异具有显著性（P〈0．05）。采用流式细胞术方法检测DDP、ADM中低剂量组可使经TAM同步化的MCF-7细胞PCD％增加，与不同步化组相比较差异具有显著性（P〈0．05）。结论不同化疗药物可诱导经TAM同步化的细胞发生凋亡。 |
| 关 键 词： | 细胞同步化 细胞凋亡 |
| 文章编号： | 1007-4287（2007）03-0384-04 |
| 收稿时间： | 2005-03-18 |
| 修稿时间： | 2005年3月18日 |
The effect of chemotherapy medicine on the survival rate and apoptosis of synchronized MCF-7 cells in culture |
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| LIU Ya-hui,LIU Bo,TAN Yan.The effect of chemotherapy medicine on the survival rate and apoptosis of synchronized MCF-7 cells in culture[J].Chinese Journal of Laboratory Diagnosis,2007,11(3):384-387. | |
| Authors: | LIU Ya-hui LIU Bo TAN Yan |
| Abstract: | Objective To study the effect of different chemotherapy medicine on the survival rate and apoptosis of synchronized MCF-7 cells in culture.Methods MTT assay and flow cytometer(FCM) assay were used to determine the survival rate and apoptosis of MCF-7 cells,which was estrogen receptor positive in culture.Results The time of MCF-7 cells to proliferate twice was 33 hours,and TAM could synchronize the MCF-7 in G0/G1 phase.When using MTT assay,it was found that 5-Fu,DDP,ADM could decrease the survival rate of MCF-7 in middle-and low-dose groups,and had a significant difference compared with the asynchronized groups(P<0.05).When using FCM assay,it was determined that DDP,ADM could increase the PCD% of MCF-7 in middle-and low-dose groups and had a significant difference compared with the asynchronized groups(P<0.05).Conclusion Different chemotherapy medicine can induce the apoptosis of the MCF-7 cells. |
| Keywords: | TAM |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
