卵巢癌患者外周血CD4~+ CD25~+调节性T细胞的检测及其临床意义

目的探讨卵巢癌患者CD4+CD25+调节性T细胞的比例变化及其可能的机制。方法利用流式细胞仪检测20例卵巢癌患者外周血淋巴细胞(peripheral blood lymphocytes,PBLs)、肿瘤浸润淋巴细胞(tumor infiltrating lymphocytes,TILs)及肿瘤相关淋巴细胞(tumor associated lymphocytes,TALs)中CD4+CD25+调节性T细胞(CD4+CD25+Tcells,Tregs)所占的比例。10例健康人外周血PBLs中Tregs所占的比例。同时检测卵巢癌患者CD4+外周血淋巴细胞中CD69+细胞的比例。健康人PBLs在含有卵巢癌细胞株SKOV3培养上清的RPMI1640培养液(有或没有血清植物血凝素)中培养72小时后,检测Tregs所占的比例。结果卵巢癌患者外周血淋巴细胞中CD4+CD25+调节性T细胞所占比例与健康人相比明显增加.卵巢癌患者外周血肿瘤浸润淋巴细胞中CD4+CD25+调节性T细胞所占比例高于在外周血淋巴细胞和肿瘤相关淋巴细胞中的比例,但是没有统计学意义。卵巢癌患者CD4+外周血淋巴细胞中无CD69表达。结论卵巢癌患者外周血淋巴细胞中、肿瘤浸润淋巴细胞及肿瘤相关淋巴细胞中CD4+CD25+调节性T细胞所占的比例增加,这可能与卵巢癌细胞分泌的可溶性分子上调有关。

The detection of CD4+ CD25+ regulatory T cells of ovarian cancer patients and clinical significance

Objective To study the frequence of the CD4＋CD25＋ regulatory in the patients with ovarian carcinoma and its possible mechanism.Methods The percentages of CD4＋CD25＋ T cells in the peripheral blood lymphocytes（PBLs）,tumorinfiltrating lymphocytes（TILs） and tumor associated lymphocytes（TALs） from 13 patients with ovarian carcinoma and in the PBLs from 14 healthy women were determined by flow cytometry.The expression of CD69 on CD4＋PBLs from thepatients was detected.PBLs from healthy women were cultured in complete RPMI 1640 containing the supernatant from SKOV3 cell line with or without PHA（phytohemagglutinin） stimulationfor 72 hours,then the percentage of CD4＋CD25＋ T cells was detected.Results CD4＋CD25＋ T cells in the PBLs from patients with ovarian carcinoma were significantly increased compared with the control.The percentage of CD4＋CD25＋ T cells in TILs was higher than that in PBLs and TALs from the patients,but not significantly.The expression of CD69 on CD4＋PBLs from the patients was negative.Conclusion There is an increasing of the proportion of CD4＋CD25＋ T cells in PBLs,TILs and TALs of the patients with ovarian carcinoma,which probably results from up-regulation ofsoluble factor secreted by ovarian carcinoma cells.