坐骨神经损伤后脊髓肝细胞生长因子mRNA及蛋白的表达

目的 研究坐骨神经损伤后再生过程中脊髓肝细胞生长因子（HGF）mRNA和蛋白的表达及经时变化规律，探讨周围神经损伤的机制。方法 采用原位杂交及免疫组化技术检测坐骨神经损伤后再生过程中脊髓HGF的mRNA及蛋白的表达。结果 大鼠坐骨神经损伤模型损伤侧的神经细胞胞质颗粒阳性染色强于未损伤侧；神经损伤后第3，7和14天，感觉。运动和副交感神经元内杂交信号明显增强，以第7天的变化最为显著。HGF蛋白的表达均于坐骨神经损伤后第1周开始增强，第2周时达峰值，然后下降。结论 周围神经损伤后，内源性HGF mRNA和蛋白表达增强，对神经元具有保护作用。

Messenger RNA and protein expression of spinal cord hepatocyte growth factor after sciatic nerve injury

Objective To study hepatocyte growth factor(HGF) mRNA and protein expression in the spinal cord during regeneration after sciatic nerve injury. To investigate the mechanism of peripheral nerve injury. Methods Seventy-four Wistar rats were divided into three groups: a regeneration after nerve injury group (injury group), a sham injury group and a normal control group. Crash injury or sham injury was induced of the sciatic nerve on the right side of the animals in the injury group or the sham injury group by using forceps. In situ hybridization and immunohistochemistry techniques were used to detect HGF mRNA and protein expression in the spinal cord during the course of regeneration after sciatic nerve injury had been induced. Results Compared with the uninjured side, positive dyeing of cytoplasmic granules on the injured side of the spinal cord was obviously enhanced. Clearly enhanced hybridization signals were observed at the 3rd, 7th and 14th day after injury, especially at the 7th day. Enhanced expression of HGF protein began in the 1st week and peaked in the 2nd week after sciatic nerve injury, then began to descend. Conclusion Endogenous HGF mRNA production and protein expression increased, and it plays a protective role after peripheral nerve injury.