miR-320及下游靶基因survivin在银屑病皮损中的表达

目的检测寻常性银屑病皮损中miR-320及其下游靶基因survivin mRNA的表达水平。方法采用实时定量聚合酶链反应(RT-PCR)检测40例寻常性银屑病患者皮损及40名健康对照皮肤中miR-320、survivin mRNA的表达水平,运用双荧光素酶报告实验验证survivin是miR-320的靶基因。miR-320和survivin表达水平两组间比较采用t检验,寻常性银屑病皮损中miR-320与survivin表达的相关性采用Pearson相关分析。结果与健康对照皮肤相比,寻常性银屑病皮损中miR-320表达量为对照组的(0.561±0.11)倍,表达明显下调(t=3.06,P0.05);survivin mRNA表达量为对照组的(2.034±0.26)倍,表达水平明显上调(t=3.35,P0.05);双荧光素酶报告实验结果提示survivin是miR-320的靶基因,寻常性银屑病皮损中miR-320与survivin mRNA呈负相关(r2=0.634,P0.05)。结论 miR-320可能通过调控其下游靶基因survivin的异常表达参与寻常性银屑病皮损的形成过程。

Expressions of miR-320 and its downstream target gene survivin in psoriatic lesions

Objective To measure the expressions of miR-320 and its downstream target gene survivin in psoriatic vulgaris lesions. Methods Tissue specimens were collected from lesions of 40 patients with psoriasis vulgaris and normal skin of 40 healthy human controls. Real-time PCR was performed to detect miR-320 and survivin mRNA expressions. Luciferase reporter assay was used to evaluate whether survivin is a direct target of miR-320. Statistical analysis was carried out by t-test for intergroup comparisons and by Pearson correlation analysis for the analysis of correlation between miR-320 and survivin expressions in psoriasis vulgaris lesions. Results Compared with the normal control skin, psoriasis vulgaris lesions showed significantly decreased mRNA expression level of miR-320 (0.561±0.1l, t=3.06, P<0.05), but increased mRNA expression level of survivin (2.034±0.26, t=3.35, P<0.05). The mRNA expression level of miR-320 showed a signiifcantly negative correlation with that of survivin (r2=0.634, P<0.05). Luciferase reporter assay showed that survivin was a direct target of miR-320. Conclusion MiR-320 might participate in the development of psoriatic lesions via targeting the regulation of its downstream target survivin.