HIV-1与HIV-2交叉反应毒株的基因亚型分析

目的分析HIV-1+2蛋白印迹试验中HIV-1确证阳性同时出现HIV-2带型样本的基因亚型。方法将HIV-1+2确证试验中同时出现HIV-2带型的样本,通过基因诊断确认是否为HIV-2共感染;同时扩增HIV-1 gag,env基因段,通过测序和系统进化树的构建,分析具有HIV-2带型的HIV-1毒株的基因亚型。结果在34份出现HIV-2带型的标本中,基因诊断发现均为交叉反应,并非HIV-1和HIV-2共感染。出现交叉反应的毒株中以AE亚型为主,占73.5%(25/34),BC重组和B亚型分别占17.7%(6/34)和8.8%(3/34)。结论HIV-1+2蛋白确证试验中HIV-1确证阳性,同时出现HIV-2带型的阳性样本,并非HIV-1和HIV-2共感染,而是交叉反应。出现交叉反应的HIV-1毒株以AE亚型为主。

The Gene Subtype of Strain in the Cross Reaction of HIV-1 and HIV-2

Objective To study the gene subtype of strain in the cross reaction of HIV-1 and HIV-2. Methods Collected the HIV-1+2 confirmatory test samples with HIV-2 band, to confirm if HIV-2 infection through the gene diagnostic. Amplificated HIV-1 env and gag gene by PCR;Studied the HIV-1 gene subtype with HIV-2 cross reaction by Sequencing and polygenetic analysis. Results These samples with HIV-1 and HIV-2 band were not HIV-1 and HIV-2 co-infection but cross reaction with HIV-2, Studied the env and gag gene of HIV-1 with HIV-2 cross reaction found that AE subtype was 73.5% (25/34); BC subtype was 17.7 %(6/34); B subtype was 8.8%(3/34); had no other subtype was found in these samples. Conclusion Most HIV-1+2 confirmatory test samples with HIV-2 bands were not HIV-1 and HIV-2 co-infection, but HIV-1 and HIV-2 cross reaction. The AE subtype of HIV-1 was mainly with cross reaction in this research.