葡萄糖通过mTORC1信号通路调控成纤维细胞增殖

目的 研究葡萄糖对小鼠皮肤成纤维细胞增殖的影响.方法 将小鼠皮肤成纤维细胞培养在含不同浓度葡萄糖的培养基里,用MTT法检测细胞增殖,7-MGTP pulldown实验检测细胞翻译起始情况,Western blot检测mTORC1信号通路分子的活化.结果 与培养基葡萄糖浓度为5.5 mmol/L相比较,当浓度为15 mmol/L时,促进细胞增殖,与7-MGTP结合的翻译起始复合物增加,mTORC1信号通路活化；当25 mmol/L时,抑制细胞增殖,与7-MGTP结合的翻译始复合物减少,mTORC1信号通路中与细胞增殖相关的4EBP1和与细胞生存相关的Akt的磷酸化减弱.结论 葡萄糖通过对mTORC1信号通路的双向调节作用调控成纤维细胞增殖.

Glucose controls the fibroblast proliferation through mTORC1 signal pathway

Object To study the effect of glucose on the mouse skin fibroblast proliferation. Methods The cells cultured in different concentrations of glucose medium including 5.5mmol/L、15mmol/L、25mmol/L, and tested the cells proliferation by MTT. The formation of translation initiation factor 4F complex was analyzed by 7-MGTP pulldown test and the mTORC1 signal pathway was analyzed by Western blot. Result In contrasted with 5.5mmol/L, when the concentration of glucose medium was 15mmol/L, it promoted the cell proliferation and the translation initiation, activated the mTORC1 signal pathway. In contrasted with 5.5mmol/L, 25mmol/L inhibited the cell proliferation and the translation initiation, inactivated the mTORC1 signal pathway (included the 4EBP1 related with cell proliferation and the Akt related with cell survival), but the S6K1 was over activatied. Conclusion Glucose controls the fibroblast proliferation through the mTORC1 signal pathway.