双特异性抗体对LAK细胞增殖和细胞毒作用影响的体外研究

将抗ＣＤ３与抗ＨＢｓ的单克隆抗体经化学偶联得到双特异性抗体，观察该双特异性抗体对ＬＡＫ细胞增殖和增强细胞毒性的作用。结果显示双特异性抗体显著提高ＬＡＫ细胞与２．２．１５细胞结合率；促进淋巴细胞增殖。１２５Ｉ－ＵｄＲ释放试验检测发现双特异性抗体增强ＬＡＫ细胞对２．２．１５细胞的细胞毒性且与抗体浓度呈正相关。进一步对抗体的特异性研究表明，加入双特异性抗体后ＬＡＫ细胞对２．２．１５细胞毒性作用显著高于对照组。

Study of the effect of bispecific antibody on proliferation and cytotoxicity of LAK cells

We observed the regulatory effects of BsAb ,chemical conjugate of CD3McAb and HBs-McAb ,on the proliferation and cytotoxicity of LAK cells in vitro The results showed that BsAb significantly increased the conjugate formation between LAK and 2. 2. 15 cells and proliferation of lymphocytes . The cytotoxic activity of LAK cells was determined by 125 I-UdR release assay. The results indicated that the BsAb could result in concentration dependent enhancement effects on the cytotoxicity against 2. 2. 15 cells. When the effectors to targets ratio was 50:1 the intensity of cytotoxicity was 70. 63% at a concentration of 1ug/ml of BsAb. The cytotoxicity of LAK cells would increase when the ratio of effectors to targets rose up. The cytotoxicity of LAK cells for 2. 2. 15 cells was compared with K 562 cells. The cytotoxic response had no significant difference if BsAb had not been added. When BsAb was at 1ug/ml the cytotoxicity of LAK cells against 2. 2. 15 cells reached 72.69% . The cytotoxicity to HepG-2 and K 562 cells was still at 42. 01% ,44. 8%. The results clearly Showed that BsAb could specifically combine LAK cells with 2. 2. 15 cells and selectively increase the cytotoxicity of LAK cells.