酒精诱导大鼠脂肪肝变TNF-α、IL-6的表达

目的观察酒精诱导大鼠脂肪肝组织中肿瘤坏死因子-α（Tumor Necrosis Factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）水平的变化。方法以56％酒精灌胃，造成大鼠酒精性脂肪肝模型，取血清测丙氨酸转氨酶（alanine aminotransferase，ALT）、门冬氨酸氨基转氨酶（aspartate aminotransferase，AST）活性，取肝组织做免疫组织化学检测TNF-α、IL-6表达。结果模型组大鼠毛发光泽差，食欲差，大便溏泻，体重增长缓慢（P〈0．01），模型组大鼠肝组织出现不同程度的肝脂肪变性，血清中ALT、AST活性较正常组明显升高，差异有统计学意义（P〈0．01），模型组肝组织中TNF-α、IL-6蛋白阳性产物呈棕褐色细颗粒状。结论肝细胞中TNF-α、IL-6水平的升高可能是酒精性脂肪肝发生的重要机制之一。

The Expression of TNF-a and IL-6 in Ethanol-Induced Rat Model

Objective To abserve the change of TNF-α and IL-6 in the hepatic tissue of alcoholic fatty liver rats. Methods The rats with alcoholic fatty liver were given 56% alcohol by daily garage,after 12 weeks, to observer the change of serum activities of alanine aminotransferase（ALT） and aspartate aminotransferase（AST）,and the expression tumor necrosis factor（TNF）-α and interleukin （IL）-6 of hepatic tissue were observed. Results The model group rats＇livers became more fatty and activities ascending of ALT and AST than normal and there was significant difference（P〈 0. 05）. the contents of TNF-α and IL-6 in liver were higher than the normal group rats. Conclusion The increasing of TNF-a and IL-6 in liver tissue is possibly the one of the important mechanism for alcoholic fatty liver.