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Cord blood stem cell-generated KIR+NK cells effectively target leukemia cell lines |
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| Affiliation: | 1. Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran;2. Department of Histopathology and Anatomy, Faculty of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran;3. Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran;4. Department of Medical Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran;5. Department of Anatomical Sciences, Sarab Faculty of Medical Sciences, Sarab, Iran;6. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;7. Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran;8. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran |
| Abstract: | Acute lymphoid (ALL) and myeloid leukemia (AML) are known to be invasive and highly lethal hematological malignancies. Because current treatments are insufficient and have a variety of side effects, researchers are looking for new and more effective therapeutic methods. Interestingly, ongoing efforts to find the best approach to optimize NK cell anti-leukemia potential shed light on the successful treatment of cancer. Mature KIR+NK cells ability to remove HLA Class-I deficient cells has been exploited in cancer immunotherapy. Here, we generated KIR+NK cells from cord blood stem cells using IL-2 and IL-15 cytokines. Our finding underlined the importance of KIR expression in the cytotoxic function of NK cells. Taken together, this study presented an effective in vitro method for the expansion and differentiation of KIR+NK cells using cytokines without any feeder cells. Furthermore, the presented culture condition could be useful for the generation of mature and pure NK cells from limited numbers of CD34+ cord blood cells and might be used as a novel method to improve the current state of cancer therapy. |
| Keywords: | Natural Killer Cells Killer Cell Immunoglobulin-like Receptors (KIRs) Acute Lymphoblastic Leukemia Acute Myeloid Leukemia Cord Blood Stem Cells ALL"} {"#name":"keyword" "$":{"id":"pc_OEAWmZML8R"} "$$":[{"#name":"text" "_":"Acute Lymphoblastic Leukemia AML"} {"#name":"keyword" "$":{"id":"pc_X8xPlTZFCW"} "$$":[{"#name":"text" "_":"Acute Myeloid Leukemia HLA"} {"#name":"keyword" "$":{"id":"pc_lLmekrZn4Z"} "$$":[{"#name":"text" "_":"Human Leukocyte Antigen NK"} {"#name":"keyword" "$":{"id":"pc_6RF0Y42tFq"} "$$":[{"#name":"text" "_":"Natural Killer KIR"} {"#name":"keyword" "$":{"id":"pc_fBBrpBwuok"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors KIR2DL1"} {"#name":"keyword" "$":{"id":"pc_nG6ZBUxkWm"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors 2 Domain Long1 KIR2DL2"} {"#name":"keyword" "$":{"id":"pc_CyjPEK9Yy1"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors 2 Domain Long 2 KIR2DL3"} {"#name":"keyword" "$":{"id":"pc_hdep4Upxn9"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors 2 Domain Long3 KIR3DL1"} {"#name":"keyword" "$":{"id":"pc_3BRrtVmhWx"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors3 Domain Long1 MACS"} {"#name":"keyword" "$":{"id":"pc_WZNVey6vBy"} "$$":[{"#name":"text" "_":"Magnetic Activating Cell sorting MNCs"} {"#name":"keyword" "$":{"id":"pc_u6ZqLgEA0l"} "$$":[{"#name":"text" "_":"Mononuclear Cells UCB"} {"#name":"keyword" "$":{"id":"pc_JMGTkiA3Rn"} "$$":[{"#name":"text" "_":"Umbilical Cord Blood SCF"} {"#name":"keyword" "$":{"id":"pc_8otxsjO34T"} "$$":[{"#name":"text" "_":"Stem Cell Factor FLT3-L"} {"#name":"keyword" "$":{"id":"pc_TIT5GGaFSB"} "$$":[{"#name":"text" "_":"fms like Tyrosine kinase 3 Ligand IL"} {"#name":"keyword" "$":{"id":"pc_wcfTRAyqp6"} "$$":[{"#name":"text" "_":"Interleukin KIR2DS1"} {"#name":"keyword" "$":{"id":"pc_F3z4qUBSgD"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors2 Domain short1 KIR3DS1"} {"#name":"keyword" "$":{"id":"pc_bfp0HrqnOL"} "$$":[{"#name":"text" "_":"Killer Cell Immunoglobulin-like Receptors3 Domain short1 |
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