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应用基因重组病毒表达产物筛选FBL3小鼠白血病肿瘤特异性抗原表位
引用本文:秦慧莲,陈卫,陈永良,何球藻.应用基因重组病毒表达产物筛选FBL3小鼠白血病肿瘤特异性抗原表位[J].中国免疫学杂志,1997(4).
作者姓名:秦慧莲  陈卫  陈永良  何球藻
作者单位:上海医科大学免疫学教研室,美国华盛顿大学医学院肿瘤系
摘    要:肿瘤特异性抗原是肿瘤免疫研究的中心课题,众多的研究表明T细胞在肿瘤免疫中起重要作用,因而寻找T细胞所识别的肿瘤特异性表位并以此为瘤苗的靶抗原有重要的研究价值。以一系列重组F-MuLVgag病毒载体,用已建立的FBL3-CTL克隆筛选,并通过H-2I类分子结合实验,精确地测得F-MuLVgag中一个能被为FBL3-CTL克隆所识别的表位(CCLCLTVFL,p85~93)。并以此p85~93合成肽免疫动物成功诱导出能识别亲本FBL3肿瘤细胞。

关 键 词:FBL3白血病细胞  特异性表位  CTL

IDENTIFICATION OF A GAG ENCODED CYTOTOXIC T LYMPHCYTE EPITOPE FROM FBL3 LEUKEMIA VIRUS INDUCED TUMORS
Qin Huilian,Chen Wei,Chen Yongliang et al.IDENTIFICATION OF A GAG ENCODED CYTOTOXIC T LYMPHCYTE EPITOPE FROM FBL3 LEUKEMIA VIRUS INDUCED TUMORS[J].Chinese Journal of Immunology,1997(4).
Authors:Qin Huilian  Chen Wei  Chen Yongliang
Affiliation:Qin Huilian,Chen Wei,Chen Yongliang et al.Shanghai Medical University,Department of Immunology,Shanghai 200032
Abstract:FBL3 is a highly immunogenic murine leukemia of C57BL/6 origin induced by friend murine leukemia virus(MuLV).A series of FBL3 specific CD8 CTL(FBL3 CTL) clone were generated from C57BL/6 mice immunized by FBL3.By using a series of recombinant vaccinia viruses expressing F MuLV gag gene,the antigenic epitope recognized by FBL3 CTL,was localized to the leader sequence was identified as CCLCLTVFL(positions 85 93) by examining lysis of targets incubated with a series of synthetic peptides.The identity of CCLCLTVFL as the target peptide was validated by showing that immuniztion with the peptide elicited CTL that lysed FBL3.
Keywords:FBL3 Leukemia    Epitope    CTL  
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