| 胃癌组织精蛋白抗原GP_2的提取与初步分析鉴定 |
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| 引用本文： | 雷秀芬,王润田.胃癌组织精蛋白抗原GP_2的提取与初步分析鉴定[J].生物医学工程学杂志,1996(2). |
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| 作者姓名： | 雷秀芬 王润田 |
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| 作者单位： | 北京医科大学流行病学教研室 |
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| 摘 要： | 本研究结合超声粉碎、超速离心与ＤＥＡＥ阴离子交换届析等方法，从手术切除的人胃癌组织中提取了一种糖蛋白抗原ＧＰ２。分析结果表明ＧＰ２为一分子量在６７－１００ＫＤ范围、非均一的糖蛋白。进一步的研究表明其在生化特征等方面都与已报道的ＣＥＡ、ＣＡ１９－９及其它胃癌抗原不同。
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| 关 键 词： | 胃癌 糖蛋白 |
Isolation and Analysis of a Novel Glycoprotein of Stomach Cancer |
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| Lei Xiu fen, Wang Runtian.Isolation and Analysis of a Novel Glycoprotein of Stomach Cancer[J].Journal of Biomedical Engineering,1996(2). |
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| Authors: | Lei Xiu fen Wang Runtian |
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| Abstract: | A novel glycoprotein,named GP2 was extracted from a resected stomach cancer specimen by a combination of ultrosonication, ultrocentrifugation and DEAE-Sephadex anion-exchange chromotography in the pressence of 8M Urea. Analysis showed that GP: contained protein and neutral sugar in an approximate ratio: of 2.1:1. After separated by SDS-PAGE electrophoresis, Coomassie brilliant blue, PAS and AB staining demonstrated that GP: had a molecular weight ranging between 67,000 to 100,000. And it was of the nature of glycoprotein. |
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| Keywords: | Stomach cancer glycoprotein |
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