| PI3K/Akt通路在滋养细胞增殖中的调控机制研究 |
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| 引用本文： | 赵茵,夏曙,钟少平,程红,邹丽.PI3K/Akt通路在滋养细胞增殖中的调控机制研究[J].中国优生与遗传杂志,2011(10):72-74,83. |
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| 作者姓名： | 赵茵 夏曙 钟少平 程红 邹丽 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院妇产科;华中科技大学同济医学院附属同济医院肿瘤科; |
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| 基金项目： | 教育部博士点新教师基金资助编号:20070487174 20070487160 |
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| 摘 要： | 旨在探讨PI3K/Akt信号转导通路在滋养细胞增殖中的作用及具体调控机制。体外培养滋养细胞系EVT。应用MTT法检测不同浓度表皮生长因子（EGF）刺激后EVT的增殖情况;应用流式细胞技术检测不同处理组EVT凋亡情况。使用PI3K抑制剂LY294002处理细胞后,检测以上各项结果的变化。结果发现：1.随EGF浓度增高,EVT增殖呈现增强趋势,EGF在10ng/ml及其以上时效应明显;2.EGF能够显著降低EVT的凋亡发生;3.使用PI3K抑制剂LY294002明显逆转EGF的促进EVT增殖的效应。提示表皮生长因子可以活化滋养细胞的PI3K/Akt信号通路,进而促进细胞增殖,并且抑制其凋亡,PI3K抑制剂可以明显抑制EGF的促EVT增殖作用。
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| 关 键 词： | PI3K/Akt 表皮生长因子 绒毛外滋养细胞 |
The regulation mechanism of PI3K/Akt pathway in trophoblast proliferation |
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| ZHAO Yin,XIA Shu,ZHONG Shao-ping,CHENG Hong,ZOU Li..The regulation mechanism of PI3K/Akt pathway in trophoblast proliferation[J].Chinese Journal of Birth Health & Heredity,2011(10):72-74,83. |
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| Authors: | ZHAO Yin XIA Shu ZHONG Shao-ping CHENG Hong ZOU Li |
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| Affiliation: | ZHAO Yin1,XIA Shu2,ZHONG Shao-ping1,CHENG Hong1,ZOU Li1*.(1.Department of Obstetrics and Gynaecology,Union Hospital,Tongji Medicial College,Huazhong University of Science and Technology,Wuhan 430022,China,2.Department of Oncology,Tongji Hospital,Wuhan 430030,China) |
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| Abstract: | To examine the possible involvement of the phosphatidylinositol 3-kinase(PI3K) pathway in extravillous trophoblast(EVT) proliferation regulated by EGF.EVT cell line(TEV-1) cells were cultured in free serum with EGF.PI3K inhibitor LY294002 was used to interfere with PI3K /Akt signalling pathway.The proliferation and apoptosis of TEV-1 cells were measured by MTT and flow cytometry assay.With the EGF concentration increasing,MTT reduction shows upward tendency.MTT reduction was inhibited by LY294002 significan... |
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| Keywords: | PI3K/Akt Extravillous trophoblast(EVT) EGF |
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