IL-6、MMP-9、TNF-α在胎膜早破早产孕妇血清、羊水中的表达及意义

目的研究IL-6、MMP-9、TNF-α在未足月胎膜早破早产孕妇的血清、羊水中的含量及表达,探讨其与胎膜早破早产的关系。方法采用酶联免疫吸附法检测30例胎膜早破早产孕妇（PPROM组）与20例正常孕妇（对照组）血清和羊水中的IL-6、MMP-9、TNF-α的含量,同时进行胎膜的病理检查。结果 PPROM组母血清及羊水中IL-6、MMP-9的含量均高于对照（P〈0.05）,羊水中TNF-α的含量较对照组高（P〈0.05）。PPROM组绒毛膜羊膜炎者血清、羊水中IL-6、TNF-α（P〈0.05）、MMP-9（P〈0.01）水平均高于非绒毛膜羊膜炎者。结论孕妇血清、羊水中IL-6、MMP-9、TNF-α水平与PPROM感染引起的早产有关,检测其水平可作为PPROM良好的预测指标。

The levels and significance of IL-6,MMP-9,TNF-α in serum and amniotic fluid of preterm women with preterm premature rupture of membranes

Objective：To investigate the relationship between the levels of interleukin-6（IL-6）,Matrix metalloproteinase-9（MMP-9）,Tumor necrosis factor-α（TNF-α） in serum and amniotic fluid of preterm women with preterm premature rupture of membranes.Methods： The levels of IL-6,MMP-9,TNF-α were detected in serum and amniotic fluid of 30 cases with preterm premature rupture of membranes（PPROM group） and 20 cases of normal pregnant women（control group） by enzyme-linked immunosorbent assay（ELISA）;and all the embryo lemma were histopathologically confirmed after delivery.Results： The levels of IL-6,MMP-9 in serum and IL-6,MMP-9,TNF-α in amniotic fluid in PPROM group were significantly higher than those in control group（P0.05）.In PPROM group,the levels of IL-6,TNF-α（P0.05）,MMP-9（P0.01） in serum and amniotic fluid of women with chorioamnionitis were significantly higher than those in non-chorioamnionitis（P0.05）.Conclusion： The levels of IL-6,MMP-9,TNF-αin serum and amniotic fluid of women with PPROM are associated with the onset of preterm labor caused by infection,and their levels are valuable to identify the chorioamnionitis.