SLC7A11-AS1调控miR-429对口腔鳞癌细胞CAL-27细胞增殖、凋亡及迁移的影响

目的探讨SLC7A11-AS1对口腔鳞癌细胞CAL-27细胞增殖、凋亡及迁移的影响及分子机制.方法收集29例口腔鳞癌患者癌组织及癌旁组织;CAL-27细胞分为si-NC组、si-SLC7A11-AS1组、水SLC7A11-AS1+anti-miR-NC组、si-SLC7A11-AS1+anti-miR429组。实时荧光定暈PCR(RT-qPCR)检测SLC7A11-AS1和miR-429表达水平;平板克隆实验检测克隆形成细胞数;流式细胞术检测细胞凋亡率;Transwell检测细胞迁移数;蛋内质印迹(Western hlot)法检测蛋白表达;双荧光素酶报告实验验证SLC7A11-AS1和miR-429的靶向关系.结果与癌旁组织相比,口腔鳞癌组织中SLC7A11-AS1表达水平升高,miR-429表达水平降低(P<0.05)。抑制SLC7A11-AS1表达后,CAL-27细胞克隆形成数减少,细胞凋亡率升高,迁移细胞数减少,Bax表达水平升高,Bcl-2表达水平降低(P<0.05)。同时抑制SLC7A11-AS1和miR-429表达后,CAL-27细胞克隆形成数增加,细胞凋亡率降低,迁移细胞数增加,Bax表达水平降低,Bcl-2表达水平升高(P<0.05).结论抑制SLC7A11-AS1表达可通过上调miR-429抑制口腔鳞癌细胞CAL-27增殖及迁移,且促进CAL-27细胞凋亡.

SLC7A11-AS1 Regulates Effect of miR-429 on Proliferation,Apoptosis and Migration of Oral Squamous Cell Carcinoma Cell Line CAL-27

Objective To explore the effect of SLC7A11-AS1 on the proliferation,apoptosis and migration of oral squamous cell careinoma cell line CAL-27 and its molecular mecha-nism.Methods Totally,29 cases of oral squamous cell carcinoma patients with cancer tissues and adjacent tissues were collected.CAL-27 cells were divided into si-NC group,si-SLC7A11-AS1 group,si-SLC7A11-AS1+anti-miR-429 group,si-SLC7A11-AS1+anti-miR-429 group.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression levels of SLC7A11-AS1 and rniK429,plate cloning experiment to detect the number of clone-forming cells,flow cytometiy to detect the rate of apoptosis,Transwell to detect the number of cell migration,Western blotting to detect protein expression,and dual luciferase report experiment to verify the targeting relationship between SLC7A11-AS1 and miR-429.Results Compared with the adjacent tissue,the expression of SLC7A11-AS1 in oral squamous cell carcinoma tissue was increased,and the expression of miR-429 was decreased(P<0.05).After inhibiting the expression of SLC7A11-AS1,the number of CAL-27 cell clones was decreased,the rate of apoptosis was increased,the number of migrating cells was decreased,the expression of Bax was increased,and the expression of Bcl-2 was de-creased(P<0.05).After inhibiting the expression of SLC7A11-AS1 and miR-429 at the same time,the number of CAL-27 cell clones was increased,the rate of apoptosis was decreased,the number of migrating cells was increased,tlie expression of Bax was decreased,and the expression of Bcl-2 was increased(P<0.05).Conclusion Inhibiting the expression of SLC7A1111-AS1 can inhibit tlie proliferation and migration of oral squamous cell carciiioinaCAL-27 cells by up-regulatingmiR-429,and promote the apoptosis of CAL-27 cells.