胆碱能神经元和纤维在猫下丘脑的分布

DISTRIBUTION OF CHOLINERGIC NEURONS AND FIBERS IN THE HYPOTHALAMUS OF THE CAT

The cholinergic neurons and fibers of the hypothalamus could not be revealed successfully in the past,therefore,there has been general agreement that the hypotha- lamus is very poorly innervated by cholinergic system.In this study,choline acetyl- trans ferase (ChAT)-like immunoreactive positive neurons and fibers of the hypo- thalamus were revealed successfully by using avidin-biotin immunocytochemical me- thod.This study demonstrated for the first time that the cat hypothalamus is richly innervated by cholinergic system.We found cholinergic neurons of varying numbers in the following areas:dorsomedial hypothalamic nucleus,paraventricular nucleus, dorsal hypothalamic area,the area of the tuber cinereum surrounding ventromedial hypothalamic nucleus,lateral hypothalamic area,anterior hypothalamic area,anterior hypothalamic nucleus,parvocellular hypothalamic nucleus,tuber-mammillary nucl- eus,posterior hypothalamic area,anterior mammillary nucleus and supramammillary nucleus.There were a lot of ChAT-like positive fibers in the lateral hypothalamic area,mammillary area,dorsal hypothalamic area,paraventricular nucleus,parvocel- lular hypothalamic nucleus,the area of the tuber cinereum.Three kinds of neuron perikarya related to cholinergic system were identified in the hypothalamus of the cat:1.cholinergic perikarya;2.noncholinergic-cholinoceptive perikarya;3.choli- nergic-cholinoceptive perikarya.There were also immunoreactive positive fibers which were non-varicose and varicose.Two kinds of varicose-fibers,one with strong immunoreactivity and the other with weak immunoreactivity were distingui- shed.