碱性成纤维细胞生长因子对小鼠创伤性脑损伤后神经元凋亡及星形胶质细胞活化的影响

目的 观察碱性成纤维细胞生长因子(bFGF)对小鼠脑损伤后伤侧皮质和海马神经元凋亡及星形胶质细胞活化的影响. 方法 36只小鼠随机分为对照组、生理盐水组、bFGF组,每组各12只.采用自由落体打击装置建立脑损伤模型,分别于建模后3d和7d取脑(每时相点6只),应用免疫荧光双标和免疫印迹法检测大脑皮质、海马神经细胞凋亡因子caspase-3的变化,以及大脑皮质中胶质纤维酸性蛋白(GFAP)的表达. 结果 bFGF组皮质和海马中caspase-3的表达比生理盐水组和对照组减少(P<0.05);bFGF组皮质中GFAP表达比生理盐水组和对照组增加(P<0.05);生理盐水组与对照组的caspase-3及GFAP表达差异无统计学意义(P>0.05). 结论 bFGF能降低小鼠脑损伤后大脑皮质和海马caspase-3表达并增高大脑皮质GFAP表达,从而促进大脑皮质星形胶质细胞活化和抑制神经细胞凋亡.

Effect of basic fibrolast growth factor on neuronal apoptosis and astroglial activation after traumatic brain injury in mice

Objective To study the effect of basic fibroblast growth factor (bFGF) on neuronal apoptosis and gumnosis in cerebral cortex and hippocampus tissue in mice with traumatic brain injury (TBI). Methods The mice models of TBI were established by Feeney’s method. Thirty-six mice were divided randomly into three groups including control group, saline group and bFGF group (n=12 for each group)After 3 days and 7 days，double-labeled immunoflurescence was used to detect the apoptosis of neurons. Using double-labeled immunoflurescence and Western blotting, we observed the expressions of caspase-3 in cerebral cortex and hippocampus tissue. The expression of glial fibrillary acidic protein (GFAP) in cerebral cortex was detected by Western blotting. Results The expressions of caspase-3 of cerebral cortex and hippocampus in bFGF group were lower than that in saline group (P<0.05). The expressions of GFAP of cerebral cortex in bFGF group were higher than that in saline group(EM>P/EM><0.05), there were no difference on expression of caspase-3 and GFAP between control group and saline group(EM>P/EM>>0.05). Conclusion After traumatic brain injury, bFGF could reduce the expression of caspase-3 and inhibit the neuronal apoptosis in cerebral cortex and hippocampus, increase the expressions of GFAP and promote the activation of astrcytes in cerebr