p53蛋白表达及端粒酶活性与骨组织恶性肿瘤关系的研究

目的 探讨端粒酶活性表达及p53基因蛋白产物在骨组织恶性肿瘤发生、发展中的作用。方法 采用非放射性同位素TRAP-银染方法对37例骨组织恶性肿瘤及13例同一病人的肿瘤旁对照组织中端粒酶活性进行检测,p53蛋白表达的检测是运用微波处理的LSAB免疫组化法。结果 37例骨组织恶性肿瘤端粒酶活性检出率为83.8％(31/37);同时检测的13例同一病人的肿瘤旁对照组织均未检测到端粒酶活性;p53蛋白表达检测结果为51.3％(19/37),正常对照组织无1例阳性。其中p53蛋白表达阳性的19例中16例端粒酶活性有表达。结论 端粒酶活性表达可能是骨肿瘤恶性的一种潜在生化标志物,并且可能和p53基因一起参与骨肿瘤的发生、发展过程,并具有协同作用。

Studies of telomerase activity and p53 protein in musculoskeletal malignancies

Objective To investigate the effect of telomerase activity and p53 protein in the genesis and development of musculoskeletal malignancies. Methods Non-isotopic TRAP-silver staining was used to detect the activity of telomerase, the expression of p53 protein was analyzed in musculoskeletal malignancies with the use of immunohistochemistry. Results Telomerase activity was detected in 83.8%(31/37)of the musculoskeletal malignancies, While the adjacent tissues of tumors in simultaneously in 13 patients examined all revaled negative telomerase activity,p53 protein accumulations was found in 51.3% (19/37)of the samples and none in control tissues,16 of these 19 cases with expression of p53 protein display telomerase activities. Conclusions The telomerase activity may be a potential biochemical marker for malignant bone tumors and play an important role with p53 gene in the genesis and development of bone tumors.