肿瘤坏死因子-α诱导红细胞衰亡的研究

目的 探讨肿瘤坏死因子-α（TN F-α）在红细胞衰亡中的作用.方法 将分离的小鼠红细胞经TNF-α（1 ng/ml）处理6、12、24、48、72 h或在不同质量浓度（0.1、1、10 ng/ml）处理24h后,利用流式细胞仪检测红细胞前向散射（FSC）值的变化、红细胞膜磷脂酰丝氨酸（PS）和神经酰胺的标记率.结果 经TNF-α处理后的小鼠红细胞较对照组于24h后出现FSC减小（（81.5±1.02）％比（87.6±0.55）％,P＜0.05）,膜的PS外露水平和神经酰胺含量增加（（5.5±1.07）％比（2.7±0.17）％,（2.1±0.23）％比（0.7±0.26）％）,差异均有统计学意义（P＜0.01）,且随着时间的推移变化趋势更加明显.结论 TNF-α可诱导红细胞体积缩小,促进红细胞膜PS外露以及神经酰胺的增加,是红细胞衰亡的触发因素.

Study on tumor necrosis factor-α induced eryptosis

Objective To investigate the role of tumor necrosis factor-α （TNF-α） on eryptosis. Methods Erythrocytes isolated from mice were put under the treatment of TNF-α at the dose of lng/ml for 6, 12, 24, 48 and 72 h, or at different concentrations of 0.1, 1 and 10 ng/ml for 24 h. The forward scatter （FSC）, phosphatidylserine （PS） exposure and ceramide formation were determined by flow cytometry. Results Compared to control group, the decrease of FSC （（81.5 ± 1.02）% vs （87.6 ±0.55）%, P〈0.05）, the increasment of membrane PS exposure level and ceramide content （（5.5±1.07）% vs （2.7±0.17）%, （2.1±0.23）% vs （0.7±0.26）%, P〈0.01） were observed in erythrocyte under the treatment of TNF-α for 24 h with more obvious tendency over time. Conclusions TNF-α can trigger cell shrinkage, and promote PS exposure and ceramide formation on the membrane of erythrocyte.