携带EBV-LMP2基因的重组MVA痘苗病毒初步免疫效果

目的 观察携带EBV lmp2基因的重组痘苗病毒疫苗在小鼠体内诱导的LMP2特异性细胞免疫应答效果.方法 使用表达EBV lmp2基因的重组痘苗病毒MVA-Lac-LMP2,采用低（2＆#215;105 pfu/只）、中（2 ＆#215; 106pfu/只）、高（2 ＆#215; 107pfu/只）三个剂量组,分别于0、2周免疫BALB/c小鼠,末次免疫1周后应用IFN-γ ELISPOT方法检测小鼠脾淋巴细胞中EBV LMP2特异性CTL应答水平.结果 MVA-Lac-LMP2能诱导小鼠产生EBV LMP2特异性的CTL应答,其中,中、高剂量组小鼠CTL水平明显高于低剂量组（P≤0.01）,中、高剂量组小鼠诱导的CTL应答水平差异没有统计学意义.结论 MVA-Lac-LMP2能够在小鼠体内诱导EBV-LMP2特异性的细胞免疫,应答水平的高低与免疫剂量有关.

Preliminary study on the immunological effect with MVA vaccine of epstein-Barr virus-LMP2 gene

Objective Observe the effect of EBV-LMP2 specific cellular immune response after immunization with recombinant modified vaccinia virus ankara （MVA） vaccine MVA-Lac-LMP2.Methods Immunize BALB/c mice with recombinant MVA vaccine carrying EBV-LMP2 gene （MVA-Lac-LMP2） in three groups,which were low dosage （2 ＆#215; 105pfu/mouse）,medium dosage （2 ＆#215; 106 pfu/mouse） and high dosage（2 ＆#215; 107pfu/mouse）.The mice were immunized at weeks 0 and 2.One week after the final immunization,analyze the LMP2 specific CTL level of the mouse spleen lymphocytes with IFN-γ ELISPOT essay.Results Immunization of BALB/c mice with MVA-Lac-LMP2 could induce LMP2 specific CTL response.Which medium dosage and high dosage tends to elicit more potent immune response.Conclusion MVA-Lac-LMP2 could induce LMP2 specific CTL response in mice.The potency of immune responses is related with the dosage of immunization.