不同药物对青春前期大鼠睾丸扭转复位健侧睾丸的远期影响

目的:观察牛磺酸、丹血通(丹参联合血栓通)和灯盏花素对青春前期大鼠睾丸扭转复位后健侧睾丸的远期影响及其保护作用,比较3种药物对抗睾丸缺血再灌注损伤的保护作用。方法:64只4周龄健康雄性SD大鼠,随机分为假手术组、模型组、牛磺酸单次和连续给药组、丹血通单次和连续给药组以及灯盏花素单次和连续给药组,每组8只,建立左侧睾丸扭转复位动物模型(720°,2 h)。于术后6周处死大鼠,取右侧睾丸及附睾,检测睾丸组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、一氧化氮合酶(NOS)活性和丙二醛(MDA)含量,测定精子畸形率和精子活动率和精子浓度,行睾丸组织病理学观察。结果:与模型组相比,3个连续组的SOD活性、TAOC、精子活动率和精子浓度均增加,MDA含量和精子畸形率均降低(P0.05)。3个单次组的SOD活性和精子活动率(除灯盏花素单次组)均增加,MDA含量和精子畸形率均降低,牛磺酸单次组T-AOC和精子浓度增加(P0.05)。模型组可见生精小管退变,间质水肿,其它给药组睾丸扭转复位诱发的组织学改变明显改善。结论:青春前期大鼠单侧睾丸扭转复位后可致健侧睾丸缺血再灌注损伤,牛磺酸、丹血通和灯盏花素均对青春前期大鼠睾丸扭转复位后健侧睾丸远期功能恢复起到一定的保护作用,其作用效果牛磺酸明显优于丹血通和灯盏花素,丹血通明显优于灯盏花素,且连续给药明显优于单次给药。

Long-term effects of different drugs on contralateral testis with unilateral testicular torsion in prepubertal rats

AIM: To observe the long-term effects of taurine, Danxuetong (Salvia miltiorrhiza combined with Xueshuantong) and breviscapine on contralateral testis with unilateral testicular torsion in prepubertal rats, and to compare their protective effect on testis with ischemia reperfusion injury. METHODS: Four-week-old healthy male SD rats (n=64) were randomly divided into 8 groups: sham operation group, saline group, single-dose and successive taurine groups, single-dose and successive Danxuetong groups, and single-dose and successive breviscapine groups. The animal model of left testicular torsion reduction (720° for 2 h) was established. After 6 weeks of surgery, the right testes and epididymides from the rats were collected immediately to detect the total antioxidant capacity (T-AOC), the activity of superoxide dismutase (SOD), the activity of nitric oxide synthase (NOS) and the content of malondialdehyde (MDA). The sperm deformity, sperm viability and sperm concentration were measured, and the pathological examination of the testicular tissues was performed. RESULTS: Compared with saline group, the activity of SOD, T-AOC, sperm viability and sperm concentration were increased, while MDA content and sperm deformity were decreased in 3 successive groups. SOD activity and sperm viability (except single-dose breviscapine group) were increased, but MDA content and sperm deformity were decreased in 3 single-dose groups. T-AOC activity and sperm concentration were increased in single-dose taurine group. The saline group showed interstitial edema and degeneration of seminiferous tubules, and the rest groups showed a significant improvement in histological morphology. CONCLUSION: The contralateral testis in prepubertal rats was damaged after unilateral testicular torsion, and taurine, Danxuetong and breviscapine have long-term protective effect on the tissues under ischemia-reperfusion injury. The protective effect of Danxuetong is better than breviscapine, and taurine is the best of them. The effect of single-dose group is better than the successive application.