LPS对鼠肺微血管内皮细胞水通道蛋白-1表达及其功能的影响

目的：通过观察脂多糖（LPS）刺激对肺微血管内皮细胞水通道蛋白-1 mRNA表达和功能的影响，以探讨急性肺损伤肺水异常代谢的机制。方法： 在体外培养的大鼠肺微血管内皮细胞（RLMEC），分别使用浓度为100 μg/L、1 mg/L、10 mg/L的LPS与之孵育4 h、12 h、24 h后, 应用氚水掺入法测定RLMEC内氚水的信号强度，并用RT-PCR法测定AQP-1 mRNA的表达。结果： LPS刺激组氚水的信号强度显著低于对照组（P<0.01）；同时LPS刺激组AQP-1 mRNA的表达显著低于对照组。结论： LPS刺激RLMEC AQP-1 mRNA表达和摄水功能下降，提示AQP-1在急性肺损伤时可能与肺水代谢异常有关。

Effects of lipopolysaccharide on aquaporin-1 expression and function in rat lung microvessel endothelial cells

AIM: To determine if aquaporin-1 (AQP-1) expression and function is influenced after lipopolysaccharide (LPS) stimulation in rat lung microvessel endothelial cells and to investigate the mechanisms of lung fluid abnormal metabolism in acute lung injury. METHODS: LPS at different concentrations (100 μg/L, 1 mg/L or 10 mg/L) was used to stimulate cultivated rat lung microvessel endothelial cells in vitro at different stimulatory times (4 h, 12 h or 24 h), respectively. Tritium water permeation was conducted for determining the intracellular signal intensity in rat lung microvessel endothelial cells. The RT-PCR technique was applied for the assay of the expression of AQP-1 mRNA. RESULTS: The signal intensity of intracellular tritium water in the LPS stimulation group was less than that in normal control significantly. Compared with the normal control group (P<0.01), there was obvious decrease in the expression of AQP-1 mRNA in LPS stimulation groups. CONCLUSION: The decreased expression of AQP-1 mRNA and the weaken function of transporting water suggest that AQP-1 may play a major role in abnormal fluid transportation in acute injured lung.