| 人microRNA-146a慢病毒表达载体的构建及鉴定 |
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| 引用本文： | 李景怡,万瑛,张晋宇,邹丽云,李娜,刘婷,柴琳琳,熊锐华,吴玉章.人microRNA-146a慢病毒表达载体的构建及鉴定[J].免疫学杂志,2008,24(6):671-673. |
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| 作者姓名： | 李景怡 万瑛 张晋宇 邹丽云 李娜 刘婷 柴琳琳 熊锐华 吴玉章 |
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| 作者单位： | 第三军医大学全军免疫学研究所;第三军医大学第一附属医院风湿病中心; |
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| 基金项目： | 国家自然科学基金(30801030) |
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| 摘 要： | 目的构建针对has-miR-146a的慢病毒表达载体,并鉴定成熟has-miR-146a在细胞内表达水平。方法PCR扩增pri-miR-146a,克隆于慢病毒载体plenti-GFP中,转染293FT细胞,收获并浓缩慢病毒颗粒,感染Jurkat细胞。结果成功构建了has-miR-146a的慢病毒表达载体,建立了高效转染系统。结论建立了高效稳定表达has-miR-146a的慢病毒转染系统。
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| 关 键 词： | microRNA-146a 慢病毒表达载体 Jurkat细胞 |
Construction and function identification of recombinant lentivirus vector expressing has-miR-146a |
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| LI Jing-yi,WAN Ying,ZHANG Jin-yu,ZOU Li-yun,LI Na,LIU Ting,CAI Lin-lin,XIONG Rui-hua,WU Yu-zhang.Construction and function identification of recombinant lentivirus vector expressing has-miR-146a[J].Immunological Journal,2008,24(6):671-673. |
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| Authors: | LI Jing-yi WAN Ying ZHANG Jin-yu ZOU Li-yun LI Na LIU Ting CAI Lin-lin XIONG Rui-hua WU Yu-zhang |
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| Affiliation: | Institute of Immunology;PLA;Third Military Medical University;Chongqing 400038;China |
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| Abstract: | Objective To construct a lentivirus vector expressing microRNA (miRNA) miR-146a and transfect the vector into Jurkat cell line. Methods PrimiR-146a amplified by PCR was inserted into plenti-GFP vector,and then identified by restriction endonuclease digestion and nucleotide sequencing. Jurkat cell line was transfected with the plasmid pluG-miR-146a. The expression of miR-146a was detected by fluorescence microscopy,flow cytometry,and Real-time PCR. Results The sequence of miR-146a was contained in the constr... |
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| Keywords: | micmRNA-146a |
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