| 依维莫司对高糖诱导肾小球系膜细胞凋亡的保护作用 |
| |
| 引用本文： | 程澜.依维莫司对高糖诱导肾小球系膜细胞凋亡的保护作用[J].微循环学杂志,2011,21(1):15-18,80,83,84. |
| |
| 作者姓名： | 程澜 |
| |
| 作者单位： | 华中科技大学同济医学院附属同济医院心血管内科,武汉,430030 |
| |
| 摘 要： | 目的:观察依维莫司对高糖诱导肾小球系膜细胞(HBZY-1)凋亡的保护作用,并探讨其意义。方法:将培养HBZY-1细胞按不同葡萄糖和依维莫司浓度分组处理72h后,用磺酰罗丹明B法测量细胞OD值,计算各组细胞存活率,用Annexin V和PI双标法检测各组细胞凋亡率。结果:30mM葡萄糖处理的细胞存活率明显降低,凋亡率明显增加(均P<0.05)。不同浓度依维莫司均能提高细胞存活率(均P<0.05),其中以200ng/ml依维莫司效果最好(P<0.05);200ng/ml依维莫司具有明显抗HBZY-1细胞凋亡作用。结论:依维莫司对高糖诱导HBZY-1细胞凋亡具有保护作用,从而为其治疗糖尿病肾病提供新的研究方向。
|
| 关 键 词： | 依维莫司 肾小球系膜细胞 凋亡 |
The Protective Effect of Everolimus on High Glucose Induced Apoptosis of Mesangial Cells |
| |
| Cheng Lan.The Protective Effect of Everolimus on High Glucose Induced Apoptosis of Mesangial Cells[J].Chinese Journal of Microcirculation,2011,21(1):15-18,80,83,84. |
| |
| Authors: | Cheng Lan |
| |
| Affiliation: | Cheng Lan/Department of Cardiology,Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030 |
| |
| Abstract: | Objective:To research the protective effect of everolimus on high glucose induced the apoptosis of mesangial cells and discuss the significance. Method:We collected and divided HBZY-1 cells. After treating cells with agent for 72h,we detected OD values with SRB method to calculate the cells growth rate and detected apoptosis cells rate with flow cytometry. Results:The growth rate of mesangial cells treated with 30mMglucose was significantly decreased and the apoptosis rate was significantly increased (P<0.0... |
| |
| Keywords: | Everolimus Mesangial cell Apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
