应用胚挽救技术获得三倍体柑橘植株

以3个柑橘异源四倍体体细胞杂种(即四倍体粗柠檬与哈姆林甜橙体细胞杂种,简称"HR";酸柚与粗柠檬体细胞杂种,简称"SR";墨西哥来檬与伏令夏甜橙体细胞杂种,简称"KV")为父本,分别与二倍体单胚性沙田柚进行有性杂交,在有性胚还没有完全败育以前,通过胚抢救技术进行三倍体植株培养(以四季柚花粉亲本为对照)。结果表明,处理的杂交组合直接成苗率极显著低于对照(P0.01),其中又以沙田柚×HR组合成苗率相对较高,达10.1%;较适合瘪籽沙田柚幼胚离体培养的培养基是MT+GA31.0mg/L+蜂皇浆200mg/L+水解乳蛋白250mg/L+蔗糖30g/L+琼脂0.7%;流式细胞分析仪检测结果显示,本试验成功获得了柑橘三倍体植株。

Regenerating triploid citrus plants by embryo rescue technique

Diploid Shatian pummelo was used as the female and crossed with the allotetraploid somatic hybrids(Citrus jambhiri+C.sinensis(HR);C.grandis+Rough lemon(SR);C.aurantifolia+C.sinensis(KV))to regenerate triploid citrus plants by embryo rescue technique,using C.grandis pollen as the control.Results showed that the regenerating rate of treated sexual hybridization was significantly lower than that of the control(P<0.01),in which the corresponding value of Shatian pummelo×HR was comparably higher(the rate of seedling is 10.1%).The optimal medium for embryo rescue was solid MT medium supplemented with GA3 1.0 mg/L,queen bee oar 200 mg/L,lacialbumin hydrolysate 250 mg/L and agar 0.7%.Results of flow cytometry analysis showed that triploid citrus plants were successfully obtained in this study.