| 黄瓜胞质6-磷酸葡萄糖酸脱氢酶基因克隆及序列分析 |
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| 引用本文： | 魏跃,王永平,李为观,吴志明,张蜀宁,陈劲枫.黄瓜胞质6-磷酸葡萄糖酸脱氢酶基因克隆及序列分析[J].西北植物学报,2009,29(10). |
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| 作者姓名： | 魏跃 王永平 李为观 吴志明 张蜀宁 陈劲枫 |
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| 作者单位： | 魏跃(南京农业大学,南方蔬菜遗传改良重点开放实验室,南京,210095;江苏农林职业技术学院,江苏镇江,212400);王永平(江苏农林职业技术学院,江苏镇江,212400);李为观,吴志明,张蜀宁,陈劲枫(南京农业大学,南方蔬菜遗传改良重点开放实验室,南京,210095) |
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| 基金项目： | 国家自然基金重点项目，国家自然科学基金，国家高技术研究发展计划('863')专项经费，教育部'111'项目，国家'973'项目 |
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| 摘 要： | 根据6-磷酸葡萄糖酸脱氢酶(6-phosphogluconate dehydrogenase,6PGDH)基因的保守氨基酸序列设计简并引物,应用RT-PCR技术从黄瓜栽培种品种'北京截头'(Cucumis sativus 'Beijingjietou')叶片中获得了640 bp的特异片段,以该序列在EST数据库进行同源检索筛选,发现甜瓜EST序列AM715537.2与之高度一致,据此设计引物经RT-PCR扩增、分子克隆和序列拼接,获得了黄瓜6-磷酸葡萄糖酸脱氢酶基因全长序列,命名为Cs6PGDH(GenBank登录号FJ610345).序列分析表明,该基因全长1 829 bp,其中开放读码框(ORF)长1 488 bp编码495个氨基酸组成的多肽,编码区内无内含子存在,5'、3'端非翻译区长度各为70 bp和271 bp.Blast同源性分析显示该基因编码的氨基酸序列与拟南芥、大豆、水稻、玉米、菠菜等物种6PGDH 基因有74%以上的一致性.由于与其他物种胞质6PGDH相类似氨基酸N端都缺少长度约为40aa的转运肽,推断Cs6PGDH为黄瓜胞质6-磷酸葡萄糖酸脱氢酶基因.
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| 关 键 词： | 黄瓜 胞质6-磷酸葡萄糖酸脱氢酶 表达序列标签 克隆 |
Cloning and Sequence Analysis of Cytosolic 6-phosphogluconate Dehydrogenase Gene cDNA from Cucumber |
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| WEI Yue,WANG Yong-ping,LI Wei-guan,WU Zhi-ming,ZHANG Shu-ning,CHEN Jin-feng.Cloning and Sequence Analysis of Cytosolic 6-phosphogluconate Dehydrogenase Gene cDNA from Cucumber[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(10). |
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| Authors: | WEI Yue WANG Yong-ping LI Wei-guan WU Zhi-ming ZHANG Shu-ning CHEN Jin-feng |
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| Affiliation: | WEI Yue1,2,WANG Yong-ping2,LI Wei-guan1,WU Zhi-ming1,ZHANG Shu-ning1,CHEN Jin-feng1(1 Key Laboratory of Southern Vegetable Crop Genetics Improvement,Nanjing Agricultural University,Nanjing 210095,China,2 Jiangsu Polytechnical College of Agriculture , Forestry,Zhenjiang,Jiangsu 212400,China) |
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| Abstract: | Degenerated primers were designed according on conserved amino acids region of 6-phosphogluconate dehydrogenase(6PGDH)gene,640 bp cDNA fragment was amplifed from Cucumis sativus 'Beijingjietou' leaves through RT-PCR method,using fragment sequence as a querying probe,one highly homologous melon EST sequence AM715537.2 was obtained from EST database.The full-length of cucumber 6-phosphogluconate dehydrogenase gene,designated as Cs6PGDH(GenBank accession number FJ610345),was obtained through RT-PCR method with... |
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| Keywords: | Cucumis sativus L cytosolic 6PGDH EST clone |
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