| 凝胶层析在发酵液中分离提纯透明质酸中的应用 |
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| 引用本文： | 吴华昌,马钦元,邓静,徐静,陈哲.凝胶层析在发酵液中分离提纯透明质酸中的应用[J].生物技术通报,2009(12). |
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| 作者姓名： | 吴华昌 马钦元 邓静 徐静 陈哲 |
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| 作者单位： | 四川理工学院生物工程系,自贡,643000 |
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| 摘 要： | 采用葡聚糖凝胶G-100,以0.1 mol/L氯化钠溶液为洗脱剂,在优化的凝胶层析条件(层析柱高度30 cm、进样量2 ml、洗脱流速1 ml/4 min)下,对透明质酸发酵液进行分离纯化,得到了高纯度的透明质酸.HA提取率达到79.85%,蛋白质去除率为84.93%.
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| 关 键 词： | 透明质酸 分离提纯 凝胶层析 |
The Application of Gel Chromatography in the Separation and Purification of Hyaluronic acid from Broth |
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| Wu Huachang,Ma Qinyuan,Deng Jing,Xu Jing,Chen Zhe.The Application of Gel Chromatography in the Separation and Purification of Hyaluronic acid from Broth[J].Biotechnology Bulletin,2009(12). |
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| Authors: | Wu Huachang Ma Qinyuan Deng Jing Xu Jing Chen Zhe |
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| Abstract: | In this research, sephadex G-100 was used to isolate and purify the broth of hyaluronic acid.HA was highly purified under the following optimized conditions,0.1 mol/L NaCl as eluent;column length is 30 cm;injection volume is 2 ml;elution flow rate is 1 ml/4min.The of HA could reach 79.85% and the was 84.93%. |
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| Keywords: | Hyaluronic acid Separation and purification Gel chromatography |
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