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| 膨化对转基因豆粕CP4-EPSPS蛋白的影响 | |
| 引用本文： | 田芳,王秀敏,滕达,杨雅麟,敖长金,王建华.膨化对转基因豆粕CP4-EPSPS蛋白的影响[J].生物技术通报,2011(10). |
| 作者姓名： | 田芳 王秀敏 滕达 杨雅麟 敖长金 王建华 |
| 作者单位： | 1. 内蒙古农业大学动物科学学院,呼和浩特010018;农业部饲料生物技术重点开放实验室,北京100081 2. 农业部饲料生物技术重点开放实验室,北京100081;中国农业科学院饲料研究所基因工程研究室,北京100081 3. 内蒙古农业大学动物科学学院,呼和浩特,010018 |
| 基金项目： | 国家转基因生物新品种培育科技重大专项,转基因生物饲料检测与监测技术(2009ZX08012-012B) |
| 摘 要： | 旨在研究转基因豆粕经挤压膨化后外源蛋白的变化规律.以CP4-EPSPS多克隆抗体和转基因含量为2％的大豆标准品作为材料,建立ELISA法定量检测抗草甘膦转基因大豆CP4-EPSPS蛋白的方法.结果表明,膨化豆粕中外源蛋白含量随着温度和含水率的升高逐渐降低,ELISA法检测低限达到0.25％,可以定量检测经过加工的转基因豆粕. |
| 关 键 词： | 酶联免疫吸附法 挤压膨化 转基因豆粕 CP4-EPSPS蛋白 |
The Effects of Extrusion on CP4-EPSPS Protein in Genetically Modified Soybean Meal |
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| Tian Fang,Wang Xiumin,Teng Da,Yang Yalin,Ao Changjin,Wang Jianhua.The Effects of Extrusion on CP4-EPSPS Protein in Genetically Modified Soybean Meal[J].Biotechnology Bulletin,2011(10). | |
| Authors: | Tian Fang Wang Xiumin Teng Da Yang Yalin Ao Changjin Wang Jianhua |
| Affiliation: | Tian Fang1,2 Wang Xiumin2,3 Teng Da2,3 Yang Yalin2,3 Ao Changjin1 Wang Jianhua2,3(1College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,2Key Laboratory of Feed Biotechnology,Ministry of Agriculture,Beijing 100081,3 Gene Engineering Laboratory,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081) |
| Abstract: | Variation tendency of CP4-EPSPS protein in genetically modified(GM) soybean via extrusion was tested in this study.The indirect ELISA assay was used to determine the CP4-EPSPS protein content with polyclonal antibody.An ELISA standard curve was created,and the LOD of ELISA was 0.25 %.The results indicated that the content of CP4-EPSPS protein decreased gradually with the increasing of temperature and moisture.Therefore,this method could be applied to detect the processed GM soybean. |
| Keywords: | Enzyme-linked immunosorbent assay Extrusion Genetically modified soybean meal CP4-EPSPS protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
