超声影像分析在高脂血症金黄地鼠模型构建中的动态监测

目的应用超声影象分析评价高脂血症金黄地鼠模型在不同时间点肝脏、主动脉弓部和心脏功能的变化,进一步探讨超声影象分析对高脂血症金黄地鼠不同时期病变的诊断价值。方法 32只叙利亚金黄地鼠分为正常对照组和高脂血症模型组,分别给以正常饲料和高脂饲料,第0、3、6、9、14、16、20、24、40周时监测血脂水平,第20和40周时各组金黄地鼠行超声影象分析和病理学检查。结果高脂饲料喂饲后金黄地鼠血清TC、TG、HDL-C、LDL-C水平显著上升（P〈0.05）,随着高脂喂饲时间的延长,不同时间点主动脉弓部内膜-中膜厚度显著增加（P〈0.000 1）,超声影象分析和病理学结果均显示高脂喂饲金黄地鼠20周时已形成脂肪肝、主动脉弓部已形成动脉粥样硬化病变,至40周时脂肪肝和动脉粥样硬化病变加重。结论超声影象分析可应用于测量高脂血症金黄地鼠模型主动脉弓部内膜-中膜厚度,连续监测脂肪肝和主动脉弓部动脉粥样硬化病变。

Establishment and dynamic ultrasonic imaging/analysis of hamster models of hyperlipidemia

Objective To study the changes of liver, aortic wall and heart function in hypeflipide-mlc hamster at different time points by ultrasonic imaging, and evaluate the value of ultrasonic diagnosis in hamster model of hyperlipidemia. Methods Thirty-two Syrian golden hamsters were randomly divided into two groups ： the control group （ n = 8 ） was fed with normal chow and the model group ＂（ n = 8 ） fed with high fat diet. The levels of Serum lipids were analyzed after 0, 3, 6, 9, 14, 16, 20, 24, 40 weeks using commercial kits. Hamsters from each group were examined by ultrasound and then sacrificed for pathological examination at the 20th or 40th weeks. Results The levels of serum TC, TG, HDL-C and LDL-C were remarkably increased in hamsters fed with high fat diet and maintained significantly above the normal levels throughout the study course （P 〈 0. 05 ）. Compared with the control group, hamsters fed with high fat diet for 20 weeks had increased intima-media thickness （IMT） （ P 〈 0. 000 1 ） and the IMT were significantly increased when the dietary intervention period was extended to 40 weeks （ P 〈 0. 000 1 ）. Fatty liver and aortic arch atherosc]erotic lesions in the hyperlipidemic hamsters fed with high fat diet for 20 weeks were revealed by both ultrasonic imaging and pathological examination, and were further developed and aggravated during the prolonged 20 weeks. Conclusions Ultrasonic imaging can be used to measure the increased aortic arch intima-media thickness of hamsters, and can also he used to monitor the dynamic development of fatty liver and atherosclerotic changes of the aortic arch wail in hyperlipidemic hamsters.