转基因EGFP小鼠的主要脏器重量及脏器系数

目的测定转基因C57BL／6-Tg（ACTB—EGFP）1osb／J（EGFP）小鼠主要脏器重量和脏器系数。方法实验选用5～6周雄性、6～7周雌性小鼠各15只，用sartorius电子天平分别测定体重和9个主要脏器重量，计算脏器系数，并对雌雄脏器重量和脏器系数之间进行比较。结果雌雄小鼠脏器重量间比较，雄性鼠体重明显大于雌性的体重（P〈0．01）；心、肝、肺、肾、肾上腺的重量间差异极显著（P〈0．01）；脾脏比较差异显著（P〈0．05）；雌雄间脏器系数比较，肺、脑、肾上腺间差异极显著（P〈0．01），心、肝、脾、肾脏间差异不显著（P〉0．05）。结论转基因EGFP小鼠不同性别间脏器重量及脏器系数间有一定的差异，为相关研究打下了基础。

Weight of Main Organs and Organ Coefficient in EGFP Transgenic Mice

Objective To measure the organ weight and organ coefficient of C57BL/6 - Tg（ACTB-EGFP） 1osb/J mice. Methods 5 - 6 weeks male and 6 - 7weeks female 15 mice in each, were used in this experiment.The weight of various organs was measured using a Sartorius BP121S electronic balance. Results Between female and the body weight, the heart, liver, lung, kidney and adrenal weight showed very significant （ P 〈 0.01 ） ; the spleen weight difference （ P 〈 0.05） ; the coefficient of lung, brain, and adrenal showed very significant difference （P 〈 0.01 ）, but no significant difference was seen in other organ coefficient. Conclusion The weight of some main organs and organ coefficient in EGFP mice are significantly different ; the above data could be useful in the biomedical researches.