大孔树脂纯化岩高兰多酚的工艺研究

为获得大孔树脂纯化岩高兰多酚的最佳工艺,以岩高兰的地上部分为原料,通过考察6种不同类型树脂(HPD-100、X-5、AB-8、D101、HPD-600、NKA-II)的含水率、吸附率和解吸率的大小,筛选出一种最适合纯化岩高兰多酚的树脂。在此基础上,选择对纯化工艺影响较大的4种因素(上样浓度、乙醇浓度、洗脱流速、洗脱体积),进行响应面法分析得到最佳工艺。结果表明:HPD-600型大孔树脂对岩高兰多酚的纯化效果最佳,其最优工艺参数为:上样浓度0.84 mg·mL^-1;乙醇浓度62.15%;洗脱流速0.67 mL·min^-1;洗脱体积2.71 BV。该条件下,岩高兰多酚的提取率为229.18 mg·g^-1,岩高兰多酚的纯度由8.11%提高到22.56%,回收率为67.78%。本研究为岩高兰多酚的纯化工艺提供了新的技术路线,也可为岩高兰提取物的研究和应用提供参考。

Macroporous Resin Purifying Polyphenols from Empetrum nigrum Aerial Parts

In order to optimize the process of polyphenols by macroporous resin from Empetrum nigrum aerial parts,moisture content,adsorption rate and desorption rate of six resins(HPD-100,X-5,AB-8,D101,HPD-600 and NKA-Ⅱ)were investigated,and the resin with the best effect was selected.Four factors(sample concentration,ethanol concentration,elution flow rate and elution volume)that have a great influence on the purification process were selected,and the response surface method was used to analyze the optimal process.HPD-600 macroporous resin had the best purification effect on polyphenols yield.The optimal extraction conditions were as follows:sample concentration was 0.84 mg·mL^-1,ethanol concentration was 62.15%,elution flow rate was 0.67 mL·min^-1,and elution volume was 2.71 BV.Under these conditions,the yield of polyphenols was 229.18 mg·g^-1,the purity of polyphenols was increased from 8.11%to 22.56%,and the recovery rate was 67.78%.